Realization of β-lactamase as a versatile fluorogenic reporter
Realization of β-lactamase as a versatile fluorogenic reporter
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DOI：
10.1016/j.tibtech.2004.03.012
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发表时间：
2004-05-01
影响因子：
17.3
通讯作者：
Campbell, RE
中科院分区：
文献类型：
--
作者：
Campbell, RE
beta-Lactamase has emerged as the heir apparent to beta-galactosidase as a catalytic reporter for imaging biological events in live mammalian cells. In recent years, several publications have demonstrated the advantages of beta-lactamase as a reporter in applications ranging from monitoring of gene transcription to detection of protein-protein interactions. Now, Rao et al. have demonstrated that beta-lactamase can also serve as a sensitive fluorogenic reporter for imaging Tetrahymena ribozyme activity in live cells. This assay should pave the way for the screening of large libraries of ribozyme mutants by flow cytometry and, therefore, the isolation of variants with improved splicing activity in the cytoplasm of mammalian cells.
