A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection
A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection
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真菌病原体会部署一种小的沉默RNA，削弱蚊子的免疫力并促进感染
DOI：
10.1038/s41467-019-12323-1
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发表时间：
2019-09-20
影响因子：
16.6
通讯作者：
Wang, Sibao
中科院分区：
文献类型：
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作者：
Cui, Chunlai;Wang, Yan;Wang, Sibao
杀虫真菌是控制病媒生物的一种很有前途的化学杀虫剂替代品。在这里，我们证明了致病真菌球孢白僵菌输出一种类似microRNA的RNA(BBA-milR1)，通过与ArgAerte1(AGO1)结合来劫持蚊子细胞中的宿主RNA干扰机制。BBA-milR1在蚊子被真菌侵入过程中高度表达，并通过沉默蚊子Toll受体配体Spatzle4(SPZ4)的表达而抑制宿主免疫。随后，一旦进入血腔，BBA-milR1的表达就会减少，这就避免了宿主蛋白酶CLIPB9的诱导，从而激活了黑化反应。因此，我们的结果表明，病原体部署了一个跨王国的小RNA效应器，从而减弱了宿主的免疫力，促进了感染。
Insecticidal fungi represent a promising alternative to chemical pesticides for disease vector control. Here, we show that the pathogenic fungus Beauveria bassiana exports a microRNA-like RNA (bba-milR1) that hijacks the host RNA-interference machinery in mosquito cells by binding to Argonaute 1 (AGO1). bba-milR1 is highly expressed during fungal penetration of the mosquito integument, and suppresses host immunity by silencing expression of the mosquito Toll receptor ligand Spatzle 4 (Spz4). Later, upon entering the hemocoel, bba-milR1 expression is decreased, which avoids induction of the host proteinase CLIPB9 that activates the melanization response. Thus, our results indicate that the pathogen deploys a cross-kingdom small-RNA effector that attenuates host immunity and facilitates infection.