An invertebrate STING from shrimp activates an innate immune defense against bacterial infection
An invertebrate STING from shrimp activates an innate immune defense against bacterial infection
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DOI：
10.1002/1873-3468.12607
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发表时间：
2017-04-01
期刊：
影响因子：
3.5
通讯作者：
Li, Chaozheng
中科院分区：
文献类型：
--
作者：
Li, Haoyang;Wang, Sheng;Li, Chaozheng
已经提出无脊椎动物干扰素基因刺激物（STINGs）不参与对感染的先天免疫应答。在此，我们从太平洋白色对虾凡纳滨对虾（Litopenaeus vannamei，LvSTING）中鉴定了一个新的STING同源物。一些在哺乳动物中识别环状二核苷酸的关键氨基酸在LvSTING中高度保守。此外，LvSTING表达可以通过革兰氏阴性菌副溶血性弧菌（Vibrio parahaemolyticus）的攻击来稳健地诱导。LvSTING基因沉默导致抗微生物肽PEN4的表达降低，使对虾对副溶血性弧菌感染更敏感，而与重组LvSTING蛋白共注射可以在体内拯救PEN4的表达，使对虾对感染具有更强的抵抗力。总之，这些结果表明，LvSTING参与了对细菌感染的先天免疫反应。
It has been proposed that invertebrate stimulators of interferon genes (STINGs) do not take part in the innate immune response to infection. Herein, we identified a new STING homolog from pacific white shrimp Litopenaeus vannamei (LvSTING). Some amino acids crucial for recognizing cyclic dinucleotides in mammals are highly conserved in LvSTING. Moreover, LvSTING expression can be robustly induced by challenge with the Gram-negative bacteria Vibrio parahaemolyticus. Silencing of LvSTING contributes to decreased expression of the antimicrobial peptide PEN4 and renders shrimp more susceptible to V. parahaemolyticus infection, while coinjection with the recombinant LvSTING protein can rescue PEN4 expression in vivo and confer shrimp with more resistance to infection. Taken together, these results suggest that LvSTING is involved in the innate immune response to bacterial infection.