The potential mediation of nitric oxide in the activation of mitochondrion-dependent apoptosis and yak meat tenderness during postmortem aging
The potential mediation of nitric oxide in the activation of mitochondrion-dependent apoptosis and yak meat tenderness during postmortem aging
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DOI：
10.1016/j.fbio.2021.101131
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发表时间：
2021-05
期刊：
影响因子：
5.2
通讯作者：
Lin-lin Wang;R. Du;Jian Li;Z. Cai;Ling Han;Yun Mao;Y. Zhou;Qunli Yu;Lian-hong Chen
中科院分区：
文献类型：
--
作者：
Lin-lin Wang;R. Du;Jian Li;Z. Cai;Ling Han;Yun Mao;Y. Zhou;Qunli Yu;Lian-hong Chen
一氧化氮（NO）通过影响细胞凋亡途径，在多种生理和病理生理过程中发挥重要作用。然而，关于NO对细胞凋亡和牦牛肉嫩化的影响的研究报道较少。本研究旨在探讨非Ca 2+依赖性诱导型一氧化氮合酶同工酶（iNOS）来源的NO在宰后衰老过程中线粒体凋亡和肉嫩化过程中的分子机制。结果表明，盐酸氨基胍（AH）可显著降低iNOS活性和NO生成。此外，iNOS衍生的NO的抑制特性显著降低了线粒体的氧化应激程度，从而进一步减少了线粒体Ca 2+和ATP的产生。iNOS-derived NO的抑制特性还通过保护肌原纤维的降解和线粒体微观结构的破坏而影响Bcl-2家族成员、线粒体凋亡级联反应和肉嫩化。这些结果表明，iNOS来源的NO在牦牛肉老化过程中参与了线粒体凋亡途径和牦牛肉嫩化过程。
Nitric oxide (NO) may play a critical role in the regulation of various physiological and pathophysiological processes due to its effect on the apoptosis pathway. However, few reports have studied the effects of NO on apoptosis and yak meat tenderization. The present study was conducted to explore the molecular mechanisms of Ca2+-independent inducible NO synthase isozyme (iNOS)-derived NO in mitochondrial apoptosis and meat tenderization during postmortem aging. Results indicated that the aminoguanidi hydrochloride (AH) significantly reduced iNOS activities and NO production. Moreover, the inhibitory property of iNOS-derived NO dramatically decreased mitochondrial oxidative stress degree, which further reduced the production of mitochondrial Ca2+and ATP. The inhibitory property of iNOS-derived NO also influenced the Bcl-2 family members, mitochondrial apoptotic cascade reaction, and meat tenderization by protecting against the degradation of myofibrils and the damage of mitochondrial microstructure. These results demonstrated that the iNOS-derived NO possessed a significant role in the mitochondrial apoptosis pathway and yak meat tenderization during aging.