SSR-tagged breeding scheme for allogamous crops: a trial in bunching onion (Allium fistulosum)
SSR-tagged breeding scheme for allogamous crops: a trial in bunching onion (Allium fistulosum)
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DOI：
10.1007/s10681-009-9961-0
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发表时间：
2009-05
期刊：
影响因子：
1.9
通讯作者：
H. Tsukazaki;K. Yamashita;A. Kojima;T. Wako
中科院分区：
文献类型：
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作者：
H. Tsukazaki;K. Yamashita;A. Kojima;T. Wako
在一些自花受精和无性繁殖的作物中，DNA 标记已被用于品种鉴定。然而，由于每个品种内的高度遗传异质性，同种异体作物如束洋葱（Allium fistulosumL.）难以进行标记辅助品种鉴定以及杂交种子纯度测试。为了帮助品种鉴定并确保洋葱分束的准确性，我们在之前的研究中提出了“SSR标记育种”方案。这里使用带有两个或四个选定 SSR 标记标记的两个群体的地方品种束洋葱来研究该方案的可行性。与对照群体相比，SSR标记群体的农艺性状没有检测到显着差异。每个种群内的目标 SSR 位点在遗传上是一致的，而其他位点则保持高度异质性。这些结果表明，SSR 标记的育种方案，即使使用非常少量的标记，对于新育成品种的鉴定也很有效，因此对于同种异体受精作物的 F1 纯度测试也很有效，其中近交衰退与成束洋葱一样严重。
In several autogamous and vegetatively propagated crops, DNA markers have been used for cultivar identification. However, allogamous crops such as bunching onion (Allium fistulosumL.) are recalcitrant to marker-aided cultivar identification, as well as hybrid seed purity tests, due to the high degree of genetic heterogeneity within each cultivar. To aid cultivar identification and ensure its accuracy in bunching onion, we proposed the “SSR-tagged breeding” scheme in our previous study. The feasibility of this scheme was investigated here using a landrace of bunching onion with two populations tagged with two or four selected SSR markers. Compared with a control population, no significant differences were detected in the agronomic traits of the SSR-tagged populations. The targeted SSR loci were genetically uniform within each population whereas other loci maintained high heterogeneity. These results demonstrate that the SSR-tagged breeding scheme, even with a very small number of markers, is efficient for the identification of newly bred cultivars, and consequently for F1purity tests, in allogamous crops in which inbreeding depression is as severe as in bunching onion.