Graphitic Carbon Nitride Nanosheets-Based Ratiometric Fluorescent Probe for Highly Sensitive Detection of H2O2 and Glucose
Graphitic Carbon Nitride Nanosheets-Based Ratiometric Fluorescent Probe for Highly Sensitive Detection of H2O2 and Glucose
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DOI：
10.1021/acsami.6b11207
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发表时间：
2016-12-14
影响因子：
9.5
通讯作者：
Yu, Ru-Qin
中科院分区：
文献类型：
--
作者：
Liu, Jin-Wen;Luo, Ying;Yu, Ru-Qin
石墨碳氮化物（g-C3 N4）纳米片是一种新兴的石墨烯类碳基纳米材料，具有高荧光性和大比表面积，在生物传感器方面具有巨大的应用潜力。目前基于g-C3 N4纳米片的荧光生物传感器主要依赖于通过纳米片与金属离子之间的荧光猝灭相互作用的单个荧光强度阅读。在这里，我们报告的第一次开发的一种新的g-C3 N4纳米片为基础的比率荧光传感策略的H2 O2和葡萄糖的高灵敏度检测。在辣根过氧化物酶（HRP）存在下，H2 O2氧化邻苯二胺（OPD），氧化产物通过氢键和π-π堆积作用组装在g-C3 N4纳米片上，有效地猝灭了g-C3 N4的荧光，并产生了新的发射峰。比率信号变化实现了H2 O2的稳健和灵敏检测。在葡萄糖氧化酶催化葡萄糖转化为H2 O2的基础上，还开发了基于g-C3 N4的比率荧光传感平台用于葡萄糖的测定。该方法对H2 O2的检测限为50 nM，对葡萄糖的检测限为0.4 μ M，并成功地用于人血清中葡萄糖的检测。这种策略可以提供一个具有成本效益的，强大的，高通量的平台，用于检测各种物种涉及过氧化氢生成反应的生物医学应用。
Graphitic carbon nitride (g-C3N4) nanosheets, an emerging graphene-like carbon-based nanomaterial with high fluorescence and large specific surface areas, hold great potential for biosensor applications. Current g-C3N4 nanosheets based fluorescent biosensors majorly rely on single fluorescent intensity reading through fluorescence quenching interactions between the nanosheets and metal ions. Here we report for the first time the development of a novel g-C3N4 nanosheets-based ratiometric fluorescence sensing strategy for highly sensitive detection of H2O2 and glucose. With o-phenylenediamine (OPD) oxidized by H2O2 in the presence of horseradish peroxidase (HRP), the oxidization product can assemble on the g-C(3)N4 nanosheets through hydrogen bonding and pi-pi stacking, which effectively quenches the fluorescence of g-C3N4 while delivering a new emission peak. The ratiometric signal variations enable robust and sensitive detection of H2O2. On the basis of the glucose converting into H2O2 through the catalysis of glucose oxidase, the g-C3N4-based ratiometric fluorescence sensing platform is also exploited for glucose assay. The developed strategy is demonstrated to give a detection limit of 50 nM for H2O2 and 0.4 mu M for glucose, at the same time, it has been successfully used for glucose levels detection in human serum. This strategy may provide a cost-efficient, robust, and high-throughput platform for detecting various species involving H2O2-generation reactions for biomedical applications.