THE 3-PRIME TERMINAL SEQUENCE OF ESCHERICHIA-COLI 16S RIBOSOMAL RNA COMPLEMENTARITY TO NONSENSE TRIPLETS AND RIBOSOME BINDING SITES
THE 3-PRIME TERMINAL SEQUENCE OF ESCHERICHIA-COLI 16S RIBOSOMAL RNA COMPLEMENTARITY TO NONSENSE TRIPLETS AND RIBOSOME BINDING SITES
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DOI：
10.1073/pnas.71.4.1342
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
DALGARNO L
中科院分区：
文献类型：
--
作者：
SHINE J;DALGARNO L
通过逐步降解和末端标记程序，得到e。大肠杆菌16s核糖体RNA显示为Pyd-A-C-C-U-C-C-U-U-AOH。这表明RNA的这一区域能够与mRNA相互作用，并且3 '端U-U-AOHis通过与终止子的碱基配对参与了蛋白质合成的终止。序列a - c - c - u - c - c可以识别在各种噬菌体mrna的核糖体结合位点上发现的保守序列；因此，它可能参与mRNA·30S亚基复合物的形成。
With a stepwise degradation and terminal labeling procedure the 3′-terminal sequence ofE. coli16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-AOH. It is suggested that this region of the RNA is able to interact with mRNA and that the 3′-terminal U-U-AOHis involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA·30S subunit complex.