Identification and functional analysis of the autofluorescent substance in Limonium bicolor salt glands
Identification and functional analysis of the autofluorescent substance in Limonium bicolor salt glands
复制标题
补血草盐腺自发荧光物质的鉴定及功能分析
DOI：
10.1016/j.plaphy.2015.09.007
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发表时间：
2015-12-01
影响因子：
6.5
通讯作者：
Wang, Baoshan
中科院分区：
文献类型：
--
作者：
Deng, Yunquan;Feng, Zhongtao;Wang, Baoshan
二色补血草是一种典型的盐生植物，其盐腺的功能是将多余的盐分分泌到环境中。我们观察到L.双色盐腺在紫外光（330-380 nm）激发下，显示出明显的蓝色自发荧光。本研究的目的是确定和澄清这种自发荧光物质在盐分泌中的作用。苏丹红IV染色结果表明，自荧光物质定位于盐腺的角质层。此外，在用0.1 M氢氧化铵溶液、碱和酶水解处理后，主要的自发荧光物质被鉴定为阿魏酸。使用两个突变体表现出增加（fii）和减少（fii）盐腺荧光的额外实验表明，在UV激发下的盐腺的荧光强度与表皮中阿魏酸的含量呈正相关，强烈表明盐腺中的主要自发荧光物质是阿魏酸。用叶圆片法测定了盐腺的分泌量，结果表明，单个盐腺的Na+分泌速率与角质层中阿魏酸的含量呈正相关，表明角质层中阿魏酸直接参与盐腺的盐分泌。(C)2015年由Elsevier Masson SAS出版。
Limonium bicolor is a typical recretohalophyte with salt glands for the secretion of excess salts into the environment. We observed that L. bicolor salt glands showed obvious blue autofluorescence under UV excitation (330-380 nm). The aim of the present study was to identify and clarify a role for this autofluorescent substance in salt secretion. Sudan IV staining showed that the autofluorescent substance was localized in the cuticle of the salt glands. Moreover, the primary autofluorescent substance was identified as ferulic acid after treatment with 0.1 M ammonium hydroxide solution, alkaline and enzymatic hydrolysis. Additional experiments using two mutants exhibiting increased (fii) and decreased (fid) salt gland fluorescence indicated that the fluorescence intensity of salt glands under UV excitation was positively correlated with the content of ferulic acid in the cuticle, strongly suggesting that the primary autofluorescent substance in the salt glands was ferulic acid. Salt gland secretion was determined using leaf discs, and the results showed that the Na+ secretion rate per single salt gland was also positively correlated with the content of ferulic acid in the cuticle, suggesting that ferulic acid in the cuticle was directly involved in salt secretion of salt gland. (C) 2015 Published by Elsevier Masson SAS.