Phenotypic study of bacteria associated with the caribbean sclerosponge, Ceratoporella nicholsoni.
Phenotypic study of bacteria associated with the caribbean sclerosponge, Ceratoporella nicholsoni.
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与加勒比海硬化海绵（Ceratoporella nicholsoni）相关的细菌的表型研究。
DOI：
10.1128/aem.56.6.1750-1762.1990
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发表时间：
1990
影响因子：
4.4
通讯作者：
Colwell,RR
中科院分区：
文献类型：
--
作者：
Santavy,DL;Willenz,P;Colwell,RR
与加勒比硬海绵体 Ceratoporella nicholsoni (Hickson) 相关的异养细菌被发现存在于细胞外，并且仅限于海绵组织的中胚层区域。通过使用数值分类方法分析 158 个表型属性，记录了与海绵相关的可培养细菌的生理、代谢和形态属性。使用形态计量学方法来确定可通过所采用的方法培养的海绵相关细菌总数的比例，结果为海绵中栖息的细菌总数的 3% 至 11%。大约 78% 的可培养细菌聚集成四组或 phena，代表两种以前未描述的弧菌属、气单胞菌属和棒状或放线菌样菌种。大多数细菌是兼性厌氧菌，发酵蔗糖和岩藻糖，但不寻常的是不能发酵葡萄糖。这项研究是对与加勒比盆地海绵相关的异养细菌的首次全面研究，该地区在生物量和多样性方面被誉为拥有最多产的海绵种群。讨论了这些关联的可能意义。
Heterotrophic bacteria associated with the Caribbean sclerosponge, Ceratoporella nicholsoni (Hickson), were found to occur extracellularly and were confined to the mesohyl regions of the sponge tissue. Physiological, metabolic, and morphological attributes of the culturable bacteria associated with the sponge were recorded by using numerical taxonomy methods for the analysis of 158 phenotypic attributes. Morphometric methods were used to determine the proportion of the total sponge-associated bacteria that were culturable by the methods employed, with the results ranging from 3 to 11% of the total bacteria inhabiting the sponge. Approximately 78% of the culturable bacteria clustered into four groups or phena, representing two previously undescribed Vibrio spp., an Aeromonas sp., and a coryneform- or actinomycete-like sp. Most of the bacteria were facultative anaerobes, fermenting sucrose and fucose but unusual in an inability to ferment glucose. This study was the first comprehensive study of heterotrophic bacteria associated with a sponge from the Caribbean basin, a region reputed to contain the most prolific sponge populations, with respect to biomass and diversity. The possible significance of these associations is discussed.