Proto-pyroptosis: An Ancestral Origin for Mammalian Inflammatory Cell Death Mechanism in Drosophila melanogaster
Proto-pyroptosis: An Ancestral Origin for Mammalian Inflammatory Cell Death Mechanism in Drosophila melanogaster
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DOI：
10.1016/j.jmb.2021.167333
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发表时间：
2022-02-28
影响因子：
5.6
通讯作者：
Theopold, U.
中科院分区：
文献类型：
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作者：
Dziedziech, A.;Theopold, U.
在哺乳动物系统中，焦亡被描述为程序性细胞死亡的一种形式，在细胞破裂时通过随后释放细胞因子和免疫效应物对免疫功能很重要。这种形式的细胞死亡在哺乳动物中已经越来越明显，并且可以通过其他途径发生，然而，跨门，几乎没有证据表明存在焦亡。在这里，我们为黑腹果蝇体内免疫场景中焦亡的古老起源提供证据。晶体细胞，一种昆虫血细胞，被招募到伤口并破裂，随后以半胱天冬酶依赖的方式释放其胞质含量。这种基于炎症的程序性细胞死亡机制符合焦亡的特征，以前从未在昆虫体内免疫情景中描述过，它依赖于古老的凋亡机制来诱导原生细胞凋亡。此外，我们揭示了这些细胞中细胞死亡激活的上游关键参与者，包括凋亡小体，它可能在原始焦亡中发挥类似于炎性小体的替代作用。因此，果蝇可能是研究热亡在先天免疫系统中的功能意义的合适模型。(c) 2021提交人。Elsevier Ltd.出版。这是一篇基于CC BY许可（http://creativecommons.org/licenses/by/4.0/）的开放获取文章。
Pyroptosis has been described in mammalian systems to be a form of programmed cell death that is important in immune function through the subsequent release of cytokines and immune effectors upon cell bursting. This form of cell death has been increasingly well-characterized in mammals and can occur using alternative routes however, across phyla, there has been little evidence for the existence of pyroptosis. Here we provide evidence for an ancient origin of pyroptosis in an in vivo immune scenario in Drosophila melanogaster. Crystal cells, a type of insect blood cell, were recruited to wounds and ruptured subsequently releasing their cytosolic content in a caspase-dependent manner. This inflammatory based programmed cell death mechanism fits the features of pyroptosis, never before described in an in vivo immune scenario in insects and relies on ancient apoptotic machinery to induce protopyroptosis. Further, we unveil key players upstream in the activation of cell death in these cells including the apoptosome which may play an alternative role akin to the inflammasome in proto-pyroptosis. Thus, Drosophila may be a suitable model for studying the functional significance of pyroptosis in the innate immune system. (c) 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).