A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis
A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis
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DOI：
10.1016/j.cell.2004.11.044
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发表时间：
2005-01-28
期刊：
影响因子：
64.5
通讯作者：
Chory, J
中科院分区：
文献类型：
--
作者：
Yin, YH;Vafeados, D;Chory, J
油菜素内酯（BRs）通过质膜定位受体激酶信号调控植物的生长发育。我们之前的研究表明，一种新的蛋白质BES1在细胞核中积累，以响应br，在br调节的基因表达中发挥作用；然而，BES1调控基因表达的机制尚不清楚。在这项研究中，我们解剖了BES1亚域，并确定BES1是一种在体外和体内结合并激活BR靶基因启动子的转录因子。BES1与基本的螺旋-环-螺旋蛋白BIM1相互作用，协同结合许多br诱导启动子中的E box （CANNTG）序列。BIM1及其近亲家族成员的功能丧失和功能获得突变体表现出BR应答表型。因此，BES1定义了一类新的植物特异性转录因子，可以与BIM1等转录因子协同调控br诱导基因。
Brassinosteroids (BRs) signal through a plasma membrane-localized receptor kinase to regulate plant growth and development. We showed previously that a novel protein, BES1, accumulates in the nucleus in response to BRs, where it plays a role in BR-regulated gene expression; however, the mechanism by which BES1 regulates gene expression is unknown. In this study, we dissect BES1 subdomains and establish that BES1 is a transcription factor that binds to and activates BR target gene promoters both in vitro and in vivo. BES1 interacts with a basic helix-loop-helix protein, BIM1, to synergistically bind to E box (CANNTG) sequences present in many BR-induced promoters. Loss-of-function and gain-of-function mutants of BIM1 and its close family members display BR response phenotypes. Thus, BES1 defines a new class of plant-specific transcription factors that cooperate with transcription factors such as BIM1 to regulate BR-induced genes.