Alkaline extraction of cholecystokinin-immunoreactivity from rat brain.
Alkaline extraction of cholecystokinin-immunoreactivity from rat brain.
复制标题
从大鼠脑中碱提取胆囊收缩素免疫反应性。
DOI：
10.1016/0006-291x(80)91289-9
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发表时间：
1980
影响因子：
3.1
通讯作者：
R. Yalow
中科院分区：
文献类型：
--
作者：
S. Ryder;J. Eng;E. Straus;R. Yalow
碱性水提取剂从大鼠脑中消除的 CCK 免疫反应性是酸性或中性水提取剂消除的 CCK 免疫反应性的 2 至 4 倍。各种激素形式之间的分布似乎与提取剂无关：较大的基本形式（CCK-33 和 CCK-39）中约为 1 10；大约1 4 为C端十二肽(CCK-12)，其余为八肽(CCK-8)。相反，碱性和酸性水溶液在从相同组织中提取脑啡肽免疫反应性方面同样有效。我们目前无法解释各种提取剂在去除大脑中 CCK 免疫反应性方面的不同效率。
Alkaline aqueous extractants remove from rat brain 2 to 4 times the CCK-immunoreactivity that is removed by acidic or neutral aqueous extractants. The distribution among the various hormonal forms appears to be independent of the extractant: about 1 10 in the larger basic forms (CCK-33 and CCK-39); about 1 4 as the C-terminal dodecapeptide (CCK-12) and the remainder as the octapeptide (CCK-8). In contrast, alkaline and acidic aqueous solutions are equally efficient in extraction of enkephalin-immunoreactivity from the same tissues. We are presently unable to account for the very different efficiencies of the various extractants in removing CCK-immunoreactivity from brain.