EFFECT OF AN OSMOTIC STIMULUS ON THE SECRETION OF ARGININE VASOPRESSIN AND ADRENOCORTICOTROPIN IN THE HORSE
EFFECT OF AN OSMOTIC STIMULUS ON THE SECRETION OF ARGININE VASOPRESSIN AND ADRENOCORTICOTROPIN IN THE HORSE
复制标题
DOI：
10.1210/endo-124-6-3102
复制
发表时间：
1989-06-01
期刊：
影响因子：
4.8
通讯作者：
DONALD, RA
中科院分区：
文献类型：
--
作者：
IRVINE, CHG;ALEXANDER, SL;DONALD, RA
精氨酸加压素（AVP）在血液渗透压变化的反应中释放，也是一种假定的乙酰胆碱促生成素。然而，在生理范围内渗透产生的AVP增加是否影响ACTH分泌尚不清楚。我们研究了这个问题，使用我们独特的无创技术收集了六匹正常意识的马的垂体静脉血，这些马接受了静脉注射高渗盐水（HS; 5%, 0.07 ml/kg.cntdot.min） 45-60分钟。在HS前、HS中、HS后各40分钟，每5分钟采集一次垂体和颈静脉标本。HS期间平均血渗透压升高（P < 0.01），平均峰值升高14.8 mosmol/kg（范围+6 ~ +37 mosmol/kg）。颈静脉AVP由0.56±0.01升高（P < 0.01）。0.18 pmol/l（平均+-）扫描电镜)HS前到2.16 .+-。HS时0.86 pmol/l。HS患者颈静脉平均AVP与渗透压呈正相关（r = 0.82; P < 0.05）。平均颈静脉ACTH浓度从49 .+-升高（P < 0.01）。从HS前的9ng /l到148。HS期间和HS后平均皮质醇水平均高于基础水平（P < 0.05）。垂体AVP和ACTH浓度超过颈静脉浓度高达100倍，HS期间平均水平（均P < 0.01）和峰值水平（均P < 0.001）升高。HS时AVP和ACTH分泌呈搏动性。垂体AVP的平均值和峰值变化与ACTH的变化有显著相关性。6匹马在实验过程中，垂体ACTH和AVP浓度同步变化（P < 0.001），配对AVP和ACTH浓度高度相关（r = 0.73, n = 129对，P < 0.001）。我们得出结论：1)AVP分泌的生理变化与ACTH分泌的类似变化密切相关；2)渗透信号可能激活上位核和室旁核的大细胞神经元，可能是促肾上腺皮质激素功能的生理相关调节因子。
Arginine vasopressin (AVP) is released in response to changes in blood osmolality and is also a putative secretagogue for ACH. However, it is unclear whether osmotically generated increases in AVP in the physiological range influence ACTH secretion. We have studied this question using our unique noninvasive technique for collecting pituitary venous blood in six normal conscious horses that received an iv infusion of hypertonic saline (HS; 5%, 0.07 ml/kg.cntdot.min) for 45-60 min. Pituitary and jugular venous samples were collected every 5 min for 40 min before, during, and for 20 min after HS. During HS, mean blood osmolality rose (P < 0.01), with a mean peak increase of 14.8 mosmol/kg (range, +6-+37 mosmol/kg). Jugular AVP rose (P < 0.01) from 0.56 .+-. 0.18 pmol/liter (mean .+-. SEM) before HS to 2.16 .+-. 0.86 pmol/liter during HS. Mean jugular AVP and osmolality were correlated (r = 0.82; P < 0.05) during HS. Mean jugular ACTH concentrations increased (P < 0.01) from 49 .+-. 9 ng/liter before HS to 148 .+-. 54 ng/liter during HS, while mean cortisol levels during and after HS exceeded basal levels (P < 0.05). Pituitary AVP and ACTH concentrations exceeded jugular concentrations by up to 100-ofold, and mean (P < 0.01 for both) and peak (P < 0.001 for both) levels increased during HS. AVP and ACTH secretion during HS were pulsatile. The mean and peak changes in pituitary AVP were significantly correlated with those in ACTH. For the six horses together, pituitary ACTH and AVP concentration changes occurred synchronously during the experiment (P < 0.001), and the paired AVP and ACTH concentrations were highly correlated (r = 0.73; n = 129 pairs; P < 0.001). We conclude that 1) physiological changes in AVP secretion are closely associated with comparable changes in ACTH secretion, and 2) osmotic signals that presumably activate the magnocellular neurons of the suproptic and paraventricular nuclei may be physiologically relevant regulators of corticotrope function.