The Prevalence of Colistin Resistant Strains and Antibiotic Resistance Gene Profiles in Funan River, China
The Prevalence of Colistin Resistant Strains and Antibiotic Resistance Gene Profiles in Funan River, China
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中国府南河粘菌素耐药菌株流行情况及抗生素耐药基因谱
DOI：
10.3389/fmicb.2018.03094
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发表时间：
2018-12-18
影响因子：
5.2
通讯作者：
Zhang, Anyun
中科院分区：
文献类型：
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作者：
Tuo, Hongmei;Yang, Yanxian;Zhang, Anyun
城市河流附近的人为活动可能显著增加了抗生素耐药性的获得和传播。在这项研究中，我们调查了中国成都府南河中粘菌素耐药菌株的流行情况。共检测到18株mcr-1阳性分离株（17株大肠埃希菌和1株阴沟肠杆菌）和6株mcr-3阳性分离株（2株Veronii气单胞菌和4株Aeromonassa），而所有分离株均未检测到mcr-2、mcr-4和mcr-5基因。为进一步了解府南河水体的抗生素耐药性，检测了府南河水体中15种抗生素耐药基因（ARGs）和1类整合子基因（intI 1）的存在情况。在水样中发现了9个高频率（70-100%）的基因：sul 1、sul 2、intI 1、aac（6′）-Ib-cr、blaCTX-M、tetM、ermB、qnrS和aph（3′）-IIIa。值得注意的是，在水样中还发现了mcr-1、blaKPC、blaNDM和vanA基因，这些基因在天然河流系统中的报道很少。通过定量聚合酶链反应（qPCR）测定，水样中选定抗生素耐药基因[sul 1、aac（6′）-Ib-cr、ermB、blaCTX-M、mcr-1和tetM]的绝对丰度范围为0至6.0（log 10 GC/mL）。sul 1、aac（6′）-Ib-cr和ermB基因的绝对丰度最高，分别为5.8、5.8和6.0 log 10 GC/mL。6种抗生素耐药基因的绝对丰度在居民区排污口附近最高。研究结果表明，居民生活污水的排放可能有助于抗生素耐药基因在这条城市河流中的传播。观察到的这些基因的高水平反映了府南河抗生素耐药性污染的严重程度，这可能对公众健康构成威胁。
Anthropogenic activities near urban rivers may have significantly increased the acquisition and dissemination of antibiotic resistance. In this study, we investigated the prevalence of colistin resistant strains in the Funan River in Chengdu, China. A total of 18 mcr-1-positive isolates (17 Escherichia coli and 1 Enterobacter cloacae) and 6 mcr-3-positive isolates (2 Aeromonas veronii and 4 Aeromonas hydrophila) were detected, while mcr-2, mcr-4 and mcr-5 genes were not detected in any isolates. To further explore the overall antibiotic resistance in the Funan River, water samples were assayed for the presence of 15 antibiotic resistance genes (ARGs) and class 1 integrons gene (intI1). Nine genes, sul1, sul2, intI1, aac(6′)-Ib-cr, blaCTX-M, tetM, ermB, qnrS, and aph(3′)-IIIa were found at high frequencies (70–100%) of the water samples. It is worth noting that mcr-1, blaKPC, blaNDM and vanA genes were also found in water samples, the genes that have been rarely reported in natural river systems. The absolute abundance of selected antibiotic resistance genes [sul1, aac(6′)-Ib-cr, ermB, blaCTX-M, mcr-1, and tetM] ranged from 0 to 6.0 (log10 GC/mL) in water samples, as determined by quantitative polymerase chain reaction (qPCR). The sul1, aac(6′)-Ib-cr, and ermB genes exhibited the highest absolute abundances, with 5.8, 5.8, and 6.0 log10 GC/mL, respectively. The absolute abundances of six antibiotic resistance genes were highest near a residential sewage outlet. The findings indicated that the discharge of resident sewage might contribute to the dissemination of antibiotic resistant genes in this urban river. The observed high levels of these genes reflect the serious degree of antibiotic resistant pollution in the Funan River, which might present a threat to public health.