The Arabidopsis Receptor Kinase IRK Is Polarized and Represses Specific Cell Divisions in Roots
The Arabidopsis Receptor Kinase IRK Is Polarized and Represses Specific Cell Divisions in Roots
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DOI：
10.1016/j.devcel.2019.12.001
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发表时间：
2020-01-27
影响因子：
11.8
通讯作者：
Van Norman, Jaimie M.
中科院分区：
文献类型：
--
作者：
Campos, Roya;Goff, Jason;Van Norman, Jaimie M.
多细胞生物的发育需要组织间细胞分裂和分化的协调。在植物中，定向信号和隐式细胞极性被认为参与了这种协调；然而，细胞间信号传导、细胞极性和细胞组织之间的机制联系尚不清楚。在此，我们研究了花序和根尖受体激酶（IRK）在根系发育中的定位和功能。我们发现IRK-GFP在内胚层细胞中定位于外质膜结构域，但在其他细胞类型中定位于不同的结构域。我们的研究结果表明，IRK的定位是由邻近的细胞类型局部告知的。irk突变体在基质组织干细胞和内胚层中有过多的细胞分裂，表明irk通过抑制特定的细胞分裂来维持组织组织。我们预测IRK感知到一个方向性线索，负向调节这些细胞分裂，从而将细胞间信号和细胞极性与发育过程中定向细胞分裂的控制联系起来。
Development of multicellular organisms requires coordination of cell division and differentiation across tissues. In plants, directional signaling, and implicitly cell polarity, is proposed to participate in this coordination; however, mechanistic links between intercellular signaling, cell polarity, and cellular organization remain unclear. Here, we investigate the localization and function of INFLORESCENCE AND ROOT APICES RECEPTOR KINASE (IRK) in root development. We find that IRK-GFP localizes to the outer plasma membrane domain in endodermal cells but localizes to different domains in other cell types. Our results suggest that IRK localization is informed locally by adjacent cell types. irk mutants have excess cell divisions in the ground tissue stem cells and endodermis, indicating IRK functions to maintain tissue organization through inhibition of specific cell divisions. We predict that IRK perceives a directional cue that negatively regulates these cell divisions, thus linking intercellular signaling and cell polarity with the control of oriented cell divisions during development.