Micro computed tomography for vascular exploration.
Micro computed tomography for vascular exploration.
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DOI：
10.1186/2040-2384-2-7
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发表时间：
2010-03-05
期刊：
影响因子：
--
通讯作者：
Couffinhal T
中科院分区：
文献类型：
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作者：
Zagorchev L;Oses P;Zhuang ZW;Moodie K;Mulligan-Kehoe MJ;Simons M;Couffinhal T
在体内和体外研究中，小动物的血管探查需要具有非常高空间分辨率的成像硬件，能够区分大血管和小血管。微型计算机断层成像(Micro-CT)是近年来兴起的一种用于这一目的的首选方法，它提供适合于分析、量化、验证和可视化结果的高分辨率3D体积数据。然而，微型CT的有效性可能会受到一系列因素的不利影响，包括动物的物理准备、数值量化、结果的可视化以及可能性有限的量化软件。加剧这些固有困难的是缺乏一个统一的微型CT成像标准。今天的大多数微型CT是针对特定的应用，而主要由成像硬件制造商开发的量化所需的软件工具缺乏解决更具体目标所需的详细程度。本文着重介绍了微型CT在血管探查中的能力，描述了成像方案的现状，并提供了旨在使微型CT更准确、可复制和更健壮的指南和建议。
Vascular exploration of small animals requires imaging hardware with a very high spatial resolution, capable of differentiating large as well as small vessels, in both in vivo and ex vivo studies. Micro Computed Tomography (micro-CT) has emerged in recent years as the preferred modality for this purpose, providing high resolution 3D volumetric data suitable for analysis, quantification, validation, and visualization of results. The usefulness of micro-CT, however, can be adversely affected by a range of factors including physical animal preparation, numerical quantification, visualization of results, and quantification software with limited possibilities. Exacerbating these inherent difficulties is the lack of a unified standard for micro-CT imaging. Most micro-CT today is aimed at particular applications and the software tools needed for quantification, developed mainly by imaging hardware manufacturers, lack the level of detail needed to address more specific aims. This review highlights the capabilities of micro-CT for vascular exploration, describes the current state of imaging protocols, and offers guidelines and suggestions aimed at making micro-CT more accurate, replicable, and robust.