Improved attachment of mesenchymal stem cells on super-hydrophobic TiO2 nanotubes
Improved attachment of mesenchymal stem cells on super-hydrophobic TiO2 nanotubes
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DOI：
10.1016/j.actbio.2008.04.004
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发表时间：
2008-09-01
影响因子：
9.7
通讯作者：
Schmuki, Patrik
中科院分区：
文献类型：
--
作者：
Bauer, Sebastian;Park, Jung;Schmuki, Patrik
采用阳极氧化的方法在钛表面生长出直径从15 nm到100 nm的垂直取向的二氧化钛纳米管自组织层。这些二氧化钛纳米管层表现出超亲水行为。在用自组装单分子膜(十八烷基膦酸)包覆纳米管后，纳米管呈现出从疏水(108+/-2度)到超疏水(167+/-2度)的随直径变化的润湿行为。研究和比较了未修饰和修饰纳米管层上间充质干细胞的细胞黏附、铺展和生长。我们发现，在超疏水范围内，细胞的黏附和增殖受到强烈影响。纤维连接蛋白、I型胶原和层粘连蛋白等细胞外基质蛋白以及牛血清白蛋白在涂层和未涂层表面的吸附对润湿行为有很大的影响，并与管径有关。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Self-organized layers of vertically orientated TiO2 nanotubes providing defined diameters ranging from 15 up to 100 nm were grown on titanium by anodic oxidation. These TiO2 nanotube layers show super-hydrophilic behavior. After coating TiO2 nanotube layers with a self-assembled monolayer (octadecylphosphonic acid) they showed a diameter-dependent wetting behavior ranging from hydrophobic (108 +/- 2 degrees) up to super-hydrophobic (167 +/- 2 degrees). Cell adhesion, spreading and growth of mesenchymal stem cells on the unmodified and modified nanotube layers were investigated and compared. We show that cell adhesion and proliferation are strongly affected in the super-hydrophobic range. Adsorption of extracellular matrix proteins as fibronectin, type I collagen and laminin, as well as bovine serum albumin, on the coated and uncoated surfaces showed a strong influence on wetting behavior and dependence on tube diameter. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.