STEROID DESMOLASE SYNTHESIS BY EUBACTERIUM-DESMOLANS AND CLOSTRIDIUM-CADAVARIS
STEROID DESMOLASE SYNTHESIS BY EUBACTERIUM-DESMOLANS AND CLOSTRIDIUM-CADAVARIS
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DOI：
10.1128/aem.52.5.1153-1156.1986
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发表时间：
1986-11-01
影响因子：
4.4
通讯作者：
LOCASCIO, S
中科院分区：
文献类型：
--
作者：
BOKKENHEUSER, VD;WINTER, J;LOCASCIO, S
在两种专性厌氧菌中证实了类固醇链解酶的合成：一种新的细菌物种，从猫粪便中分离的链解真杆菌，和从纽约市的污水中回收的尸体梭菌。该酶切割在C-17和C-21具有羟基官能团的皮质激素的C.sbd.17-C.sbd.20键。转化是定量的，条件是底物浓度小于100 μ g/ml并且生物体处于对数期。转化速度与细菌生长曲线平行，在对数生长期E. desmolans比C.尸体此外，两种生物体都合成20 β-羟基类固醇脱氢酶
The synthesis of a steroid desmolase was demonstrated in two obligate anaerobes: a new bacterial species, Eubacterium desmolans, isolated from cat fecal fora, and Clostridium cadaveris, recovered from sewage of New York City. The enzyme cleaves the C.sbd.17-C.sbd.20 bond of corticoids possessing hydroxyl functions at C-17 and C-21. The conversion is quantitative, provided the substrate concentration is less than 100 .mu.g/ml and the organisms are in the log phase. The velocity of transformation parallels the bacterial growth curve and in the log phase is higher for E. desmolans than for C. cadaveris. In addition, both organisms synthesize a 20.beta.-hydroxysteroid dehydrogenase.