Investigation of Pneumocystis jirovecii colonization in patients with chronic pulmonary diseases in the People's Republic of China.
Investigation of Pneumocystis jirovecii colonization in patients with chronic pulmonary diseases in the People's Republic of China.
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中华人民共和国慢性肺部疾病患者耶氏肺孢子菌定植情况调查
DOI：
10.2147/copd.s89666
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发表时间：
2015
影响因子：
2.8
通讯作者：
An CL
中科院分区：
文献类型：
--
作者：
Wang DD;Zheng MQ;Zhang N;An CL
在没有肺炎体征或症状的个人的呼吸道标本中检测到吉罗夫西氏肺孢子虫DNA被定义为定植。日本血吸虫定植在各种肺部疾病发生发展中的作用已有报道，但在中国人民共和国鲜有关于日本血吸虫在患者体内定植的信息。目的：了解慢性阻塞性肺疾病(COPD)急性期和稳定期、间质性肺疾病、囊性纤维化和慢性支气管扩张症等肺部疾病患者中肺炎支原体的定植情况。建立了环介导等温扩增(LAMP)和常规聚合酶链式反应(PCR)检测日本血吸虫的方法。这项研究纳入了98名HIV阴性患者，他们接受了随访，并接受了支气管镜检查，以诊断各种潜在的呼吸道疾病。用LAMP扩增法对这些患者的唾液进行分析。此外，所有标本均采用常规聚合酶链式反应、Giemsa和Gomori‘s亚甲胺硝酸银染色方法。灵敏度和特异性试验表明，LAMP检测日本血吸虫特异性基因与其他真菌或细菌无交叉反应，最低检出限为50拷贝/毫升。在98份痰标本中，LAMP法检出62份(63.3%)，常规PCR法检出22.45%(22/98)。然而，Giemsa和Gomori的亚甲胺硝酸银检测在所有基因阳性的标本中均未发现日本血吸虫包囊。本研究结果表明，中国地区慢性肺部疾病患者中日本血吸虫定殖率较高，LAMP法较常规聚合酶链式反应更能准确地评价痰标本中日本血吸虫的定植情况。
The detection of Pneumocystis jirovecii DNA in respiratory specimen from individuals who do not have signs or symptoms of pneumonia has been defined as colonization. The role of P. jirovecii colonization in the development or progression of various lung diseases has been reported, but little information about P. jirovecii colonization in patients is available in the People’s Republic of China. To determine the prevalence of P. jirovecii colonization in patients with various pulmonary diseases, including the acute and stable stage of COPD, interstitial lung diseases, cystic fibrosis, and chronic bronchiectasis. A loop-mediated isothermal amplification (LAMP) and a conventional polymerase chain reaction (PCR) method for detecting P. jirovecii were developed. Ninety-eight HIV-negative patients who were followed-up and who had undergone bronchoscopy for diagnosis of various underlying respiratory diseases were included in the study. Sputa of these patients were analyzed with LAMP amplification of P. jirovecii gene. In addition, conventional PCR, Giemsa and Gomori’s methenamine silver nitrate staining assays were applied to all specimens. The sensitivity and specificity test showed that there was no cross-reaction with other fungi or bacteria in detecting the specific gene of P. jirovecii by LAMP, and the minimum detection limits by LAMP was 50 copies/mL. P. jirovecii DNA was detected in 62 of 98 (63.3%) sputa specimens by LAMP assay and 22.45% (22/98) by conventional PCR. However, no P. jirovecii cysts were found by Giemsa and Gomori’s methenamine silver nitrate in all of gene-positive specimens. The results of our study showed that prevalence of P. jirovecii colonization is particularly high in patients with chronic pulmonary diseases in the People’s Republic of China, and the LAMP method is better for evaluation of the colonization of P. jirovecii in sputum specimen than conventional PCR.