Investigation of signaling molecules and metabolites found in crustacean hemolymph via in vivo microdialysis using a multifaceted mass spectrometric platform.
Investigation of signaling molecules and metabolites found in crustacean hemolymph via in vivo microdialysis using a multifaceted mass spectrometric platform.
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DOI：
10.1002/elps.201500497
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发表时间：
2016-04
期刊：
影响因子：
2.9
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Jiang S;Liang Z;Hao L;Li L
神经递质是一类内源性的信号分子，在动物的多种生理过程中起着重要的调节作用。这些化学信使的检测通常具有挑战性，因为它们的浓度水平低，体外降解速率快。为了解决这些挑战，在此，我们采用体内微透析（MD）采样研究甲壳动物模型Cancer borealis中的神经递质。多方面的分离工具，如毛细管电泳（CE）和离子迁移质谱法在这项工作中使用。通过不同的机制分离小分子，并通过基质辅助激光解吸/电离质谱成像（MALDI-MSI）进行检测。还将该基于分离的MSI平台的性能与液相色谱-电喷雾电离质谱（LC-ESI-MS）进行了比较。利用MALDI和ESI MS，共鉴定了208种小分子神经递质和代谢产物，其中39种被鉴定为体内分泌的信号分子。此外，使用CE的亚微米级样品消耗的固有特性使得MD样品收集时间更短。与LC-ESI-MS相比，体内MD的时间分辨率提高了约10倍，表明应用分离辅助MALDI MS成像平台的显著优势。
Neurotransmitters (NTs) are endogenous signaling molecules which play an important role in regulating various physiological processes in animals. Detection of these chemical messengers is often challenging due to their low concentration levels and fast degradation rate in vitro. In order to address these challenges, herein we employed in vivo microdialysis (MD) sampling to study neurotransmitters in the crustacean model Cancer borealis. Multi-faceted separation tools, such as capillary electrophoresis (CE) and ion mobility mass spectrometry were utilized in this work. Small molecules were separated by different mechanisms and detected by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). Performance of this separation-based MSI platform was also compared to liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). By utilizing both MALDI and ESI MS, a total of 208 small molecule neurotransmitters and metabolites were identified, of which 39 were identified as signaling molecules secreted in vivo. In addition, the inherent property of sub micro-scale sample consumption using CE enables shorter time of MD sample collection. Temporal resolution of in vivo MD was improved by approximately 10-fold compared to LC-ESI-MS, indicating the significant advantage of applying separation-assisted MALDI MS imaging platform.