Children and adults with acute lymphoblastic leukaemia have similar gene expression profiles
Children and adults with acute lymphoblastic leukaemia have similar gene expression profiles
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DOI：
10.1111/j.1600-0609.2005.00433.x
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发表时间：
2005-06-01
影响因子：
3.1
通讯作者：
Blennow, E
中科院分区：
文献类型：
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作者：
Kuchinskaya, E;Heyman, M;Blennow, E
目的：比较儿童和成人急性淋巴细胞白血病（ALL）的基因表达模式，以提高我们对疾病生物学和预后差异的理解。方法：使用 Affymetrix 生产的寡核苷酸芯片 Hu95ver2a 分析 29 名儿童和 15 名成人 ALL 诊断样本中的基因表达谱。结果：无监督的层次聚类分析显示，尽管结果存在差异，但患者的聚类与年龄无关，首先根据 T 细胞或 B 前体免疫表型，其次根据 B 前体组内的细胞遗传学变化。表达模式分析允许将一些样本重新分类到适当的细胞遗传学组中。我们还表明，具有 BCR/ABL 易位的样本的单独聚类可以通过 BCR 中的不同断点区域来解释。 B-前体组内有和没有 CDKN2A 缺失的样本之间没有观察到基因表达的显着差异。对不同年龄组的分析显示，当比较患有 MLL 易位的婴儿和 40 岁以上的成年人时，无论核型如何，表达谱都有相似性。结论：尽管临床结果存在差异，但儿童和成人 ALL 的基因表达模式非常相似，并且主要取决于免疫表型和细胞遗传学畸变。然而，当比较年龄组时，婴儿和40岁以上成年人的表达模式显示出显着的相似性。
Objectives: To compare the gene expression pattern in children and adults with acute lymphoblastic leukaemia (ALL) in order to improve our understanding of the difference in disease biology and prognosis. Methods: The gene expression profiles in diagnostic samples from 29 children and 15 adults with ALL were analysed using the oligonucleotide chip Hu95ver2a, produced by Affymetrix. Results: Unsupervised hierarchical cluster analysis revealed that, in spite of differences in outcome, patients clustered irrespective of age, first by T-cell or B-precursor immunophenotype, and second by cytogenetic changes within the B-precursor group. The expression pattern analysis allowed the reclassification of some samples into the proper cytogenetic group. We also showed that separate clustering of samples with the BCR/ABL translocation could be explained by different breakpoint regions in the BCR. No significant difference in gene expression was observed between samples with and without CDKN2A deletion within the B-precursor group. Analysis of different age groups revealed a similarity in expression profiles when infants with the MLL translocation and adults over 40 yr of age were compared irrespective of karyotype. Conclusions: In spite of the difference in clinical outcome, the gene expression pattern in children and adults with ALL is very similar and is primarily dependent on immunophenotype and cytogenetic aberrations. However, when age groups are compared, the expression patterns of infants and adults over 40 show a remarkable similarity.