Localization of lung surfactant protein D on mucosal surfaces in human tissues
Localization of lung surfactant protein D on mucosal surfaces in human tissues
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DOI：
10.4049/jimmunol.164.11.5866
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发表时间：
2000-06-01
影响因子：
4.4
通讯作者：
Holmskov, U
中科院分区：
文献类型：
--
作者：
Madsen, J;Kliem, A;Holmskov, U
肺表面活性蛋白-D（SP-D）是一种主要由肺泡II型细胞产生的凝集素，通过与微生物碳水化合物结合启动先天免疫的效应机制。通过RT-PCR对来自人体组织的一组mRNA进行了SP-D mRNA的筛选，肺是主要合成位点，但转录物很容易从气管、脑、睾丸、唾液腺、心脏、前列腺、肾脏和胰腺中扩增。次要的合成部位是子宫、小肠、胎盘、乳腺和胃。腮腺SP-D基因与肺SP-D基因序列完全一致。制备了抗SP-D的单克隆抗体，其中一种用于通过免疫组织化学在细胞和组织中定位SP-D。SP-D免疫反应被发现在肺泡II型细胞，克拉拉细胞，肺泡巨噬细胞上和内，在腮腺，汗腺和泪腺的大小导管的上皮细胞，在胆囊和肝内胆管的上皮细胞，和外分泌胰腺导管。SP-D还存在于皮肤、食道、小肠和泌尿道的上皮细胞以及肾脏的集合管中。SP-D通常存在于粘膜表面，并且不限于肺中的细胞亚群。SP-D的定位和功能表明，该聚集蛋白是获得性免疫系统中伊加在先天免疫系统中的对应物。
Lung surfactant protein-D (SP-D), a collectin mainly produced by alveolar type II cells, initiates the effector mechanisms of innate immunity on binding to microbial carbohydrates. A panel of mRNAs from human tissues was screened for SP-D mRNA by RT-PCR, The lung was the main site of synthesis, but transcripts were readily amplified from trachea, brain, testis, salivary gland, heart, prostate gland, kidney, and pancreas. Minor sites of synthesis were uterus, small intestine, placenta, mammary gland, and stomach. The sequence of SP-D derived from parotid gland mRNA was identical with that of pulmonary SP-D. mAbs were raised against SP-D, and one was used to locate SP-D in cells and tissues by immunohistochemistry. SP-D immunoreactivity was found in alveolar type II cells, Clara cells, on and within alveolar macrophages, in epithelial cells of large and small ducts of the parotid gland, sweat glands, and lachrymal glands, in epithelial cells of the gall bladder and intrahepatic bile ducts, and in exocrine pancreatic ducts. SP-D was also present in epithelial cells of the skin, esophagus, small intestine, and urinary tract, as well as in the collecting ducts of the kidney. SP-D is generally present on mucosal surfaces and not restricted to a subset of cells in the lung. The localization and functions of SP-D indicate that this collectin is the counterpart in the innate immune system of IgA in the adaptive immune system.