Pseudomonas paralcaligenes sp. nov., isolated from a hospitalized patient
Pseudomonas paralcaligenes sp. nov., isolated from a hospitalized patient
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副产碱假单胞菌 sp.
DOI：
10.1099/ijsem.0.005649
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发表时间：
2023
影响因子：
2.8
通讯作者：
Kirikae Teruo
中科院分区：
文献类型：
--
作者：
Ono Emi;Tohya Mari;Watanabe Shin;Tada Tatsuya;Kuwahara-Arai Kyoko;Oshiba Akihiro;Izumi Namiki;Kirikae Teruo
从日本一名医院患者的粪便样本中分离出一种革兰氏染色阴性、需氧、杆状、非内生孢子形成细菌，命名为菌株MRCP 1333T。MRCP1333T在15 - 40 ° C（最佳25 - 35 ° C）的温度下生长，含1.0 - 3.0%（w/v，171 - 513 mM）NaCl [最佳1 - 2%（w/v），171 - 342 mM]，pH值为6.0 - 9.5（最佳pH值为7.0 - 8.0）。 基于16S rRNA基因和53个编码细菌核糖体蛋白亚基的基因序列的系统发育分析结果表明，MRCP1333T代表了铜绿假单胞菌类群的成员，与产碱假单胞菌的亲缘关系最近。全基因组比较，使用平均核苷酸同一性，数字DNA-DNA杂交和平均氨基酸同一性，证实了MRCP1333T代表一个独特的物种在P。航空集团表型表征试验表明，该菌株利用柠檬酸盐，甘油和d-苹果酸，能够将亚硝酸盐还原为氮，并且该菌株能够在米诺环素和四唑蓝存在下生长，从而将该菌株与P. alcaligenes和其他与P.航空集团MRCP1333T的主要脂肪酸为总特征8（C18：1 ω 7c/C18：1 ω 6c; 38.4%）、总特征3（C16：1 ω 7c/C16：1 ω 6c; 21.1%）和C16：0（20.6%）。 MRCP1333T的DNA G + C含量为66.5mol%。遗传和表型证据表明，MRCP1333T应被归类为代表一个新的物种，其名称为假单胞菌calcigenessp。十一是建议。模式菌株为MRCP1333T（= LMG 32254T，= JCM 34250T）。
A Gram-stain-negative, aerobic, rod-shaped, non-endospore-forming bacterium, designated as strain MRCP1333T, was isolated from a faecal sample from a hospital patient in Japan. MRCP1333Tgrew at temperatures of 15–40 °C (optimum 25–35 °C), with 1.0–3.0 % (w/v, 171–513 mM) NaCl [optimum 1–2 % (w/v), 171–342 mM], and at pH 6.0–9.5 (optimum pH 7.0–8.0). The results of phylogenetic analysis based on the sequences of the 16S rRNA gene and the 53 genes encoding the bacterial ribosome protein subunits indicated that MRCP1333Trepresented a member of thePseudomonas aeruginosagroup, most closely related toPseudomonas alcaligenes. Whole-genome comparisons, using average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, confirmed that MRCP1333Trepresented a distinct species in theP. aeruginosagroup. Phenotypic characterization tests demonstrated utilization by this strain of citrate, glycerol, andd-malic acid, the ability to reduce nitrite to nitrogen and the ability of this strain to grow in the presence of minocycline and tetrazolium blue, distinguishing this strain fromP. alcaligenesand other closely related species of theP. aeruginosagroup. The major fatty acids of MRCP1333Twere summed feature 8 (C18 : 1ω7c/C18 : 1ω6c; 38.4 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 21.1 %) and C16 : 0(20.6 %). The DNA G+C content of MRCP1333Twas 66.5 mol%. Genetic and phenotypic evidence indicated that MRCP1333Tshould be classified as representing a novel species, for which the namePseudomonas paralcaligenessp. nov. is proposed. The type strain is MRCP1333T(=LMG 32254T,=JCM 34250T).