Transient and persistent dendritic spines in the neocortex in vivo
Transient and persistent dendritic spines in the neocortex in vivo
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DOI：
10.1016/j.neuron.2005.01.003
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发表时间：
2005-01-20
期刊：
影响因子：
16.2
通讯作者：
Svoboda, K
中科院分区：
文献类型：
--
作者：
Holtmaat, AJGD;Trachtenberg, JT;Svoboda, K
树突棘在几天到几个月内在新皮质锥体神经元（第5层和2/3层）的顶端簇体内成像。一小部分细刺在几天内出现和消失，而大多数粗刺持续数月。在躯体感觉皮层，从出生后第16天（PND）到PND 25脊柱收缩超过增加，导致脊柱的净损失。持久刺的分数（寿命！8天）在发育过程中逐渐增长，并进入成年期（PND 16- 25，35%; PND 35- 80，54%; PND 80-120，66%; PND 175-225，73%），提供证据表明，突触回路继续稳定，即使在成人大脑中，在已知的关键期关闭后很久。在6个月大的小鼠中，与躯体感觉皮层相比，视觉中的脊椎翻转更慢，这可能反映了这些大脑区域中经验依赖性可塑性能力的差异。
Dendritic spines were imaged over days to months in the apical tufts of neocortical pyramidal neurons (layers 5 and 2/3) in vivo. A fraction of thin spines appeared and disappeared over a few days, while most thick spines persisted for months. In the somatosensory cortex, from postnatal day (PND) 16 to PND 25 spine retractions exceeded additions, resulting in a net loss of spines. The fraction of persistent spines (lifetime ! 8 days) grew gradually during development and into adulthood (PND 16-25,35%; PND 35-80,54%; PND 80-120, 66%; PND 175-225, 73%), providing evidence that synaptic circuits continue to stabilize even in the adult brain, long after the closure of known critical periods. In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions.