Survival and Environmental Stress Resistance of Cronobacter sakazakii Exposed to Vacuum or Air Packaging and Stored at Different Temperatures
Survival and Environmental Stress Resistance of Cronobacter sakazakii Exposed to Vacuum or Air Packaging and Stored at Different Temperatures
复制标题
真空或空气包装及不同温度下保存的坂崎克罗诺杆菌的生存和环境胁迫抗性
DOI：
10.3389/fmicb.2019.00303
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发表时间：
2019-02-20
影响因子：
5.2
通讯作者：
Shi, Chao
中科院分区：
文献类型：
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作者：
Bai, Yichen;Yu, Haibo;Shi, Chao
本研究的目的是评估坂崎克罗诺杆菌暴露于真空或空气包装，然后储存在 4、10 或 25°C 下的存活率，并通过将细胞置于 50°C 的重构婴儿配方奶粉中、在酸（模拟胃液，pH = 3.5）和胆汁盐 [胆汁盐溶液，5%] 中测定真空包装或空气包装细菌细胞的环境应激抵抗力。 （重量/体积）]。将无菌培养皿底部干燥的坂崎棒状杆菌的鸡尾酒培养物进行空气包装或真空包装，然后在4、10或25℃下保存10天。检查储存期间的活细胞群，并将真空包装和空气包装的细胞（在10℃下储存4天）随后暴露于热、酸或胆盐。结果表明，10天内，真空包装和空气包装的坂崎棒状杆菌的数量在4℃下分别减少了1.6和0.9 log CFU/ml，在25℃下分别减少了1.6和1.3 log CFU/ml。在 10 摄氏度时，真空包装和空气包装的细胞分别观察到显着减少 3.1 和 2.4 log CFU/ml。与空气包装相比，真空包装并在10℃下储存4天导致坂崎梭菌对热、酸和胆盐条件的抵抗力显着下降。这些结果表明，婴儿配方奶粉真空包装的应用可能有助于最大限度地降低坂崎梭菌的风险。
The aim of this study was to evaluate the survival of Cronobacter sakazakii exposed to vacuum or air packaging, then stored at 4, 10, or 25 degrees C, and the environmental stress resistance of vacuum-packaged or air-packaged bacterial cells were determined by subjecting the cells to reconstituted infant formula at 50 degrees C, in acid (simulated gastric fluid, pH = 3.5), and in bile salt [bile salt solution, 5% (wt/vol)]. A cocktail culture of C. sakazakii desiccated on the bottom of sterile petri plates was air-packaged or vacuum-packaged and then stored at 4, 10, or 25 degrees C for 10 days. The viable cell populations during storage were examined, and the vacuum-packaged and air-packaged cells (stored at 10 degrees C for 4 days) were subsequently exposed to heat, acid, or bile salt. The results show that the populations of vacuum-packaged and air-packaged C. sakazakii were reduced by 1.6 and 0.9 log colony-forming units (CFU)/ml at 4 degrees C and by 1.6 and 1.3 log CFU/ml at 25 degrees C, respectively, in 10 days. At 10 degrees C, significant reductions of 3.1 and 2.4 log CFU/ml were observed for vacuum-packaged and airpackaged cells, respectively. Vacuum packaging followed by storage at 10 degrees C for 4 days caused significant decreases in the resistance of C. sakazakii to heat, acid, and bile salt conditions compared with air packaging. These results suggest that the application of vacuum packaging for powdered infant formula could be useful to minimize the risk of C. sakazakii.