Three-dimensional ultrastructure of chloroplast pockets formed under salinity stress
Three-dimensional ultrastructure of chloroplast pockets formed under salinity stress
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盐胁迫下叶绿体袋的三维超微结构
DOI：
10.1111/pce.13115
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Taniguchi Mitsutaka
中科院分区：
文献类型：
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作者：
Yamane Koji;Oi Takao;Enomoto Sakiko;Nakao Tomoyo;Arai Shigeo;Miyake Hiroshi;Taniguchi Mitsutaka
我们研究了叶绿体的内陷结构，它包围着叶绿体基质薄层中的线粒体和过氧化物酶体等细胞器，这层基质被称为叶绿体口袋。在本研究中，在盐胁迫的水稻植株中观察到叶绿体口袋，但在中等生长条件下没有观察到。它们包括细胞质、透明结构、脂质体、线粒体和过氧化物酶体。我们通过使用透射电子显微镜和聚焦离子束扫描电子显微镜获得的系列图像构建了叶绿体口袋的三维结构。透射电镜观察到3种类型的叶绿体袋：细胞器完全封闭在叶绿体袋中（封闭型）、叶绿体袋中部有小间隙（间隙型）和叶绿体袋一侧开口（开口型）。在连续成像观察到的70个囊袋中，35个为封闭型，21个为间隙型，14个为开放型。线粒体和过氧化物酶体经常与叶绿体口袋接触。聚焦离子束扫描电子显微镜显示叶绿体具有部分包围过氧化物酶体的片状结构。这一事实表明，叶绿体可能会构建大的片状结构，这将与叶绿体口袋的形成。
We investigated the invagination structure of a chloroplast that surrounds organelles such as mitochondria and peroxisomes within a thin layer of chloroplast stroma, which is called a chloroplast pocket. In this study, chloroplast pockets were observed in rice plants subjected to salinity stress but not under moderate growth condition. They included cytosol, transparent structure, lipid bodies, mitochondria, and peroxisomes. We constructed the three‐dimensional architecture of chloroplast pockets by using serial images obtained by transmission electron microscopy and focused ion beam‐scanning electron microscopy. Three types of chloroplast pockets were observed by transmission electron microscopy: Organelles were completely enclosed in a chloroplast pocket (enclosed type), a chloroplast pocket with a small gap in the middle part (gap type), and a chloroplast pocket with one side open (open type). Of the 70 pockets observed by serial imaging, 35 were enclosed type, and 21 and 14 were gap and open types, respectively. Mitochondria and peroxisomes were often in contact with the chloroplast pockets. Focused ion beam‐scanning electron microscopy revealed chloroplasts with a sheet structure partially surrounding peroxisomes. This fact suggests that chloroplasts might construct large sheet structures that would be related to the formation of chloroplast pockets.