Variable microsatellite loci isolated from the Asian honeybee, Apis cerana (Hymenoptera; Apidae)
Variable microsatellite loci isolated from the Asian honeybee, Apis cerana (Hymenoptera; Apidae)
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DOI：
10.1111/j.1755-0998.2009.02268.x
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发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Yoshida, Tadaharu
中科院分区：
文献类型：
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作者：
Takahashi, Jun-Ichi;Shimizu, Souta;Yoshida, Tadaharu
本研究利用磁粒法对亚洲蜜蜂Apis cerana进行了12个微卫星座位的扩增。这12个基因中有8个是高度多态的，有4到7个等位基因，预期杂合度在0.38到0.78之间。这些引物还在相关蜜蜂物种中产生多态产物，如黑腹蜜蜂。这些基因座可以用来研究与遗传结构相关的参数，如父子关系频率和工人繁殖。
We developed 12 polymorphic microsatellite loci for the Asian honeybee, Apis cerana using the magnetic particle method. Eight of these 12 were highly polymorphic, having four to seven alleles with an expected heterozygosity of 0.38 to 0.78. The primers also produce polymorphic products in related honeybee species such as Apis nigrocincta. These loci can be used to study parameters associated with genetic structure, such as paternity frequency and worker reproduction.