EFFECTS OF FISH-OIL ON RECTAL CELL-PROLIFERATION, MUCOSAL FATTY-ACIDS, AND PROSTAGLANDIN-E2 RELEASE IN HEALTHY-SUBJECTS
EFFECTS OF FISH-OIL ON RECTAL CELL-PROLIFERATION, MUCOSAL FATTY-ACIDS, AND PROSTAGLANDIN-E2 RELEASE IN HEALTHY-SUBJECTS
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DOI：
10.1016/0016-5085(93)90135-y
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发表时间：
1993-11-01
期刊：
影响因子：
29.4
通讯作者：
KASPER, H
中科院分区：
文献类型：
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作者：
BARTRAM, HP;GOSTNER, A;KASPER, H
背景：实验研究表明，饮食中的鱼油在结肠癌的发生中具有保护作用。前列腺素被认为参与了这一过程。本文研究了鱼油对12名健康志愿者直肠细胞增殖(即肿瘤风险的中间生物标志物)、粘膜脂肪酸和前列腺素E2(PGE2)释放的影响。方法：在双盲交叉试验中，受试者在控制基础饮食的基础上，补充鱼油(每天4.4gω-3脂肪酸)或玉米油补充剂，为期两个4周。用溴脱氧尿嘧啶核苷免疫组织化学方法和鸟氨酸脱羧酶活性检测直肠细胞增殖。与溴脱氧尿嘧啶核苷孵育2小时后，用放射免疫法测定孵育液中PGE2的浓度。用气相色谱分析粘膜脂肪酸。结果：鱼油中溴脱氧尿苷标记指数(9.2%vs.10.9%；P<；0.005)、鸟氨酸脱羧酶活性(19.7pmol·mg Protein−1.h−1；P<；0.005)和直肠活检组织PGE2释放量(671.5 pg/mg湿组织)均显著低于玉米油期(P<；0.05)，膜脂肪酸差异无统计学意义。结论：研究结果支持膳食鱼油可预防结肠癌的假说。
Background: Experimental studies have indicated dietary fish oil as a protective agent in colon carcinogenesis. Prostaglandins have been suggested to be involved in this process. In the present study, the effects of fish oil on rectal cell proliferation (ie, intermediate biomarker of cancer risk), mucosal membrane fatty acids, and prostaglandin E 2 (PGE 2) release were investigated in 12 healthy volunteers. Methods: In addition to a controlled basal diet, the test subjects received either fish oil (4.4 g ω-3 fatty acids/day) or corn oil supplements for two 4-week periods in a double-blind, crossover trial. Rectal cell proliferation was determined by bromodeoxyuridine immunohistochemistry and ornithine decarboxylase activity. After 2-hour incubation with bromodeoxyuridine, PGE 2 concentration in the incubation medium was measured by radioimmunoassay. Mucosal membrane fatty acids were analyzed by gas chromatography. Results: Bromodeoxyuridine labeling index (9.2% vs. 10.9%; P< 0.05), ornithine decarboxylase activity (19.7 vs. 36.4 pmol· mg protein− 1· h− 1; P< 0.005), and PGE 2 release from rectal biopsy specimens (435.5 vs. 671.5 pg/mg wet tissue; P< 0.05) were significantly lower during the fish oil than the corn oil period, whereas membrane fatty acids were not statistically different. Conclusions: The results support the hypothesis that dietary fish oil may protect against colon cancer.