Mushroom (Agaricus bisporus) polyphenoloxidase inhibited by apigenin: Multi-spectroscopic analyses and computational docking simulation
Mushroom (Agaricus bisporus) polyphenoloxidase inhibited by apigenin: Multi-spectroscopic analyses and computational docking simulation
复制标题
芹菜素抑制蘑菇（双孢蘑菇）多酚氧化酶：多光谱分析和计算对接模拟
DOI：
10.1016/j.foodchem.2016.02.045
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发表时间：
2016-07-15
期刊：
影响因子：
8.8
通讯作者：
Chen, Jun
中科院分区：
文献类型：
--
作者：
Xiong, Zhiqiang;Liu, Wei;Chen, Jun
已有研究表明，某些多酚类物质可以防止多酚氧化酶(PPO)引起的酶促褐变。芹菜素是一种重要的生物活性黄酮类化合物，广泛存在于多种水果和蔬菜中。在本研究中，芹菜素对PPO表现出很强的抑制活性，一些试剂与芹菜素具有协同抑制PPO的作用。芹菜素以混合型方式可逆地抑制PPO活性。PPO的失活速率常数(K)增大，活化能(E-a)和热力学参数(Delta G、Delta H和Delta S)减小，说明PPO的热敏性和稳定性降低。荧光发射光谱和圆二色谱揭示了PPO的构象变化。原子力显微镜观察表明，与芹菜素作用后，PPO分子的尺寸变大。此外，计算对接模拟表明，芹菜素与PPO结合，并插入到PPO的疏水空腔中与一些氨基酸残基相互作用。(C)2016爱思唯尔有限公司。保留所有权利。
It has been revealed that some polyphenols can prevent enzymatic browning caused by polyphenoloxidase (PPO). Apigenin, widely distributed in many fruits and vegetables, is an important bioactive flavonoid compound. In this study, apigenin exhibited a strong inhibitory activity against PPO, and some reagents had synergistic effect with apigenin on inhibiting PPO. Apigenin inhibited PPO activity reversibly in a mixed-type manner. The fact that inactivation rate constant (k) of PPO increased while activation energy (E-a) and thermodynamic parameters (Delta G, Delta H and Delta S) decreased indicated that the thermosensitivity and stability of PPO decreased. The conformational changes of PPO were revealed by fluorescence emission spectra and circular dichroism. Atomic force microscopy observation suggested that the dimension of PPO molecules was larger after interacting with apigenin. Moreover, computational docking simulation indicated that apigenin bound to PPO and inserted into the hydrophobic cavity of PPO to interact with some amino acid residues. (C) 2016 Elsevier Ltd. All rights reserved.