Coupled external cavity photonic crystal enhanced fluorescence.
Coupled external cavity photonic crystal enhanced fluorescence.
复制标题
DOI：
10.1002/jbio.201200173
复制
发表时间：
2014-05
影响因子：
2.8
通讯作者：
Cunningham BT
中科院分区：
文献类型：
--
作者：
Pokhriyal A;Lu M;Ge C;Cunningham BT
我们报告了一种增强荧光的全新方法，其中表面吸附的荧光团标记的生物分子在光子晶体表面上被激发，该光子晶体表面作为窄带宽和可调谐的外腔激光反射镜。与普通光子晶体增强荧光相比，该方案使电磁增强因子提高了约10倍。在我们的实验中，腔体自动调整其激光波长到光子晶体的共振波长，即使在存在可变器件参数和表面吸附捕获分子密度变化的情况下，也能确保最佳的共振耦合。与在无图案玻璃基板上的检测相比，我们在荧光团标记蛋白的检测极限上实现了~105倍的改进。增强的荧光信号和容易的光学对准使得腔耦合光子晶体成为进一步降低用于生物测定的光激发光发射器检测限的可行方法。
We report a fundamentally new approach to enhance fluorescence in which surface adsorbed fluorophore-tagged biomolecules are excited on a photonic crystal surface that functions as a narrow bandwidth and tunable mirror of an external cavity laser. This scheme leads to ~10× increase in the electromagnetic enhancement factor compared to ordinary photonic crystal enhanced fluorescence. In our experiments, the cavity automatically tunes its lasing wavelength to the resonance wavelength of the photonic crystal, ensuring optimal on-resonance coupling even in the presence of variable device parameters and variations in the density of surface-adsorbed capture molecules. We achieve ~105× improvement in the limit of detection of a fluorophore-tagged protein compared to its detection on an unpatterned glass substrate. The enhanced fluorescence signal and easy optical alignment make cavity-coupled photonic crystals a viable approach for further reducing detection limits of optically-excited light emitters that are used in biological assays.