Molecular relatedness of ESBL/AmpC-producing Escherichia coli from humans, animals, food and the environment: a pooled analysis
Molecular relatedness of ESBL/AmpC-producing Escherichia coli from humans, animals, food and the environment: a pooled analysis
复制标题
DOI：
10.1093/jac/dkx397
复制
发表时间：
2018-02-01
影响因子：
5.2
通讯作者：
Heederik, Dick J. J.
中科院分区：
文献类型：
--
作者：
Dorado-Garcia, Alejandro;Smid, Joost H.;Heederik, Dick J. J.
背景：近年来，产ESBL/ ampc的大肠杆菌（ESBL/AmpC-EC）越来越多地从动物、食物、环境和人类中分离出来。由于证据不完整和分散，这些水库对人类携带负担的贡献仍不清楚。目的：量化不同储层之间的分子相似性，作为风险归因的第一步。方法：从荷兰的35项研究中收集ESBL/AmpC-EC分离株的汇总数据，包括：27000份样本，大部分在2005年至2015年期间获得。通过比例相似指数（psi）和主成分分析（pca）比较了22个菌株5808株ESBL/AmpC基因的频率分布和812株ESBL/AmpC携带质粒的复制子。结果：ESBL/AmpC基因在各基因库中均有优势表达。PCAs和psi结果显示，人类养殖群体与动物（肉鸡和猪）之间的ESBL/AmpC基因具有密切的人-动物相似性（psi值为0.8 ~ 0.9）。从一般人群中分离出来的病毒与从人类临床环境、地表水和污水以及野生鸟类中分离出来的病毒具有较高的相似度（0.7-0.8），而与牲畜或食物库的相似度较低（0.3-0.6）。稀疏曲线显示，普通人群在ESBL/AmpC基因和质粒复制子类型上的多样性高于其他库。结论：我们的“同一个健康”方法提供了来自众多来源的ESBL/AmpC-EC分子相关性的综合评估。分析显示，不同宿主之间的ESBL/AmpC- ec传播周期存在差异，但未能证明牲畜养殖场与普通人群之间ESBL/AmpC基因和质粒复制子类型之间存在密切的流行病学联系。
Background: In recent years, ESBL/AmpC-producing Escherichia coli (ESBL/AmpC-EC) have been isolated with increasing frequency from animals, food, environmental sources and humans. With incomplete and scattered evidence, the contribution to the human carriage burden from these reservoirs remains unclear.Objectives: To quantify molecular similarities between different reservoirs as a first step towards risk attribution.Methods: Pooled data on ESBL/AmpC-EC isolates were recovered from 35 studies in the Netherlands comprising. 27 000 samples, mostly obtained between 2005 and 2015. Frequency distributions of ESBL/AmpC genes from 5808 isolates and replicons of ESBL/AmpC-carrying plasmids from 812 isolates were compared across 22 reservoirs through proportional similarity indices (PSIs) and principal component analyses (PCAs).Results: Predominant ESBL/AmpC genes were identified in each reservoir. PCAs and PSIs revealed close human-animal ESBL/AmpC gene similarity between human farming communities and their animals (broilers and pigs) (PSIs from 0.8 to 0.9). Isolates from people in the general population had higher similarities to those from human clinical settings, surface and sewage water and wild birds (0.7-0.8), while similarities to livestock or food reservoirs were lower (0.3-0.6). Based on rarefaction curves, people in the general population had more diversity in ESBL/AmpC genes and plasmid replicon types than those in other reservoirs.Conclusions: Our 'One Health' approach provides an integrated evaluation of the molecular relatedness of ESBL/AmpC-EC from numerous sources. The analysis showed distinguishable ESBL/AmpC-EC transmission cycles in different hosts and failed to demonstrate a close epidemiological linkage of ESBL/AmpC genes and plasmid replicon types between livestock farms and people in the general population.