The HpmA hemolysin is more common than HlyA among Proteus isolates.
The HpmA hemolysin is more common than HlyA among Proteus isolates.
复制标题
在变形杆菌分离株中，HpmA 溶血素比 HlyA 更常见。
DOI：
10.1128/iai.58.6.1853-1860.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Welch,RA
中科院分区：
文献类型：
--
作者：
Swihart,KG;Welch,RA
两种不同的溶血素，HpmA和HlyA，已被报道在变形杆菌属中。为了研究这些溶血素在变形杆菌菌株中的分布，筛选了来自各种感染和正常粪便的溶血素产生菌株。所有63株奇异变形杆菌菌株和24株普通变形杆菌菌株中的23株产生了在无细胞上清液中可检测到的钙非依赖性溶血活性。钙非依赖性的活动是由于HpmA，这种活动与HpmA序列的存在和生产的细胞外166千道尔顿（kDa）的蛋白质与抗HpmA抗血清反应。hpmA-突变体，构建的hpmA基因的中心部分缺失，不产生166 kDa的蛋白质，不再溶血时，与它们各自的亲本菌株相比。在87株奇异变形杆菌和普通变形杆菌中，只有两株普通变形杆菌产生了钙依赖性溶血活性。这些菌株产生了一个110 kDa的蛋白质与大肠杆菌溶血素（HlyA）抗体的免疫印迹。这些研究表明，变形杆菌属。产生两种不同的细胞外溶血素，几乎所有菌株都产生钙非依赖性溶血素HpmA，但只有偶尔的普通疟原虫分离株产生HlyA。
Two different hemolysins, HpmA and HlyA, have been reported in Proteus spp. To study the distribution of these hemolysins among Proteus strains, isolates from various infections and normal feces were screened for hemolysin production. All 63 Proteus mirabilis strains and 23 of the 24 Proteus vulgaris strains produced a calcium-independent hemolytic activity detectable in cell-free supernatants. The calcium-independent activity was due to HpmA; this activity correlated with the presence of hpmA sequences and the production of an extracellular 166-kilodalton (kDa) protein that reacted with anti-HpmA antiserum. HpmA- mutants, constructed by deletion of the central portion of the hpmA gene, did not produce the 166-kDa protein and were no longer hemolytic when compared with their respective parent strains. Among the 87 P. mirabilis and P. vulgaris isolates examined, calcium-dependent hemolytic activity was produced by only two P. vulgaris strains. These strains produced a 110-kDa protein which comigrated with the Escherichia coli hemolysin (HlyA) antibodies in immunoblots. These studies show that Proteus spp. produce two distinct extracellular hemolysins, with nearly all strains producing the calcium-independent hemolysin, HpmA, but only an occasional P. vulgaris isolate producing HlyA.