Terrimonas suqianensis sp nov., isolated from a tetrabromobisphenol A-contaminated soil
Terrimonas suqianensis sp nov., isolated from a tetrabromobisphenol A-contaminated soil
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从四溴双酚 A 污染的土壤中分离出宿迁 Terrimonas sp nov.
DOI：
10.1007/s10482-017-0879-6
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发表时间：
2017
影响因子：
2.6
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Chen Qing;Zang Xiao-Xia;Hang Xing;Li Shun-Peng;Jiang Jian-Dong;Chen Kai;Chen Jing;Wang Hong-Mei;Chen K
从四溴双酚A污染土壤中分离到一株革兰氏阴性、无孢子、杆状细菌C3- 5 T。在10-37 °C（最佳30 °C）和pH 5.5-9.5（最佳pH 7.0）下观察到生长。基于16 S rRNA基因序列的系统发育分析表明，菌株C3- 5 T为Terrimonas属的成员，与北京Terrimonas pekingensis QHT和根球Terrimonas rootsphaerae CR 94 T的序列相似性较高（99.0%），与其它已知Terrimonas种的序列相似性较低（<97%）。化学分类学分析表明，菌株C3- 5 T含有甲基萘醌-7（MK-7）作为主要的类异戊二烯醌，含有iso-C15：0、iso-C15：1G、iso-C17：03-OH和总和特征3（C16：1ω6c和/或C16：1ω 7 c）作为主要的脂肪酸（占总脂肪酸的5%以上）。极性脂质被确定为脂质、糖脂、磷脂、磷酸氨基脂和磷脂酰乙醇胺。DNA G+C含量为42.6mol%。与近缘菌株T. pekingensisQHT和T.根际球孢菌CR 94 T分别为25.2%和48.5%。根据系统发育分析、表型特征和化学分类学数据，菌株C3- 5 T被认为是Terrimonas属的一个新种，命名为Terrimonas suqianensissp.十一是建议。模式菌株为C3- 5 T（= CCTCC AB 2017042 T = KCTC 52676 T）。
Strain C3-5T, a Gram-negative, asporogenous, rod-shaped bacterium, was isolated from a tetrabromobisphenol A contaminated soil. Growth was observed at 10–37 °C (optimum 30 °C) and at pH 5.5–9.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3-5Tis a member of the genusTerrimonasand exhibits high sequence similarities withTerrimonas pekingensisQHT(99.0%) andTerrimonas rhizosphaeraeCR94T(97.3%), and exhibits low (<97%) sequence similarities with other knownTerrimonasspecies. Chemotaxonomic analysis revealed that strain C3-5Tpossesses menaquinone-7 (MK-7) as the major isoprenoid quinone and iso-C15:0, iso-C15:1G, iso-C17:03-OH and summed feature 3 (C16:1ω6c and/or C16:1ω7c) as the major (>5% of total) fatty acids. The polar lipids were determined to be a lipid, glycolipid, phospholipid, phosphoaminolipid and phosphatidylethanolamine. The DNA G+C content was found to be 42.6 mol%. The DNA–DNA relatedness values with the closely related strainsT. pekingensisQHTandT. rhizosphaeraeCR94Twere 25.2 and 48.5%, respectively. Based on phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, strain C3-5Tis considered to represent a novel species of the genusTerrimonas, for which the nameTerrimonas suqianensissp. nov. is proposed. The type strain is C3-5T(= CCTCC AB 2017042T= KCTC 52676T).