ULTRASTRUCTURAL OBSERVATIONS ON SECONDARY MEROGONY AND GAMETOGONY OF DACTYLOSOMA-RANARUM LABBE, 1894 (EUCOCCIDIIDA, APICOMPLEXA)
ULTRASTRUCTURAL OBSERVATIONS ON SECONDARY MEROGONY AND GAMETOGONY OF DACTYLOSOMA-RANARUM LABBE, 1894 (EUCOCCIDIIDA, APICOMPLEXA)
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DOI：
10.2307/3282527
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发表时间：
1987-10-01
影响因子：
1.3
通讯作者：
DESSER, SS
中科院分区：
文献类型：
--
作者：
BARTA, JR;BOULARD, Y;DESSER, SS
本文用常规切片和冰冻切片对蛙趾体（Dactylosoma ranarum（Lankester，1871）Labbe，1894）的次级裂殖和配子发生进行了超微结构观察。次级裂殖子由周边出芽形成，在裂殖早期同时发生有丝分裂和胞质分裂。次级裂殖子有一个三层膜，微孔，圆锥形与2顶环，4棒状体，和许多微丝。支链淀粉夹杂物中偶尔观察到次级分生体和配子体阶段。Gamonts of D.蛙在宿主红细胞内弯曲，并在其核的后部折叠，以产生“尾”，该“尾”的直径约为核前区的一半，并向后延伸其前部长度的约80%。每个配子体都有一个完整的顶端复合体，包括一个圆锥体。大约50个膜下微管位于前部区域，没有发生在后部。雄性和雌性配子不能区分光学或电子显微镜。未成熟配子周围的寄生虫空泡的膜被精心制作成一个网络的膜延伸突出到邻近的红细胞质。这种扩展的冷冻断裂复制品表明，网络是连续的寄生虫空泡周围的未成熟的配子的膜，它可能与这些阶段进入红细胞的入口。
Ultrastructural observations on secondary merogony and gametogony of Dactylosoma ranarum (Lankester, 1871) Labbe, 1894, in erythrocytes of Rana esculenta were made on standard thin sections and freeze-fracture replicas. Secondary merozoites formed by peripheral budding with karyokinesis and cytokinesis occurring simultaneously during early merogony. Secondary merozoites had a trilaminate pellicle, micropores, a conoid with 2 apical rings, 4 rhoptries, and numerous micronemes. Amylopectin inclusions were observed occasionally within secondary merogonic and gametogonic stages. Gamonts of D. ranarum were recurved within the host erythrocyte and were folded posterior to their nucleus to produce a "tail" which was approximately one-half the diameter of the prenuclear region and which extended back about 80% of the length of their anterior portion. Each gamont had a complete apical complex including a conoid. Approximately 50 subpellicular microtubules were located in the anterior region; none occurred in the posterior portion. Male and female gamonts could not be distinguished by either light or electron microscopy. The membrane of the parasitophorous vacuole surrounding immature gamonts was elaborated into a network of membranous extensions projecting into the neighbouring erythrocyte cytoplasm. Freeze-fracture replicas of such extensions demonstrated that the network is contiguous with the membrane of the parasitophorous vacuole surrounding immature gamonts and that it may be related to the entrance of these stages into the erythrocyte.