DIFFERENTIAL REARING EFFECTS ON RAT VISUAL-CORTEX SYNAPSES .1. SYNAPTIC AND NEURONAL DENSITY AND SYNAPSES PER NEURON
DIFFERENTIAL REARING EFFECTS ON RAT VISUAL-CORTEX SYNAPSES .1. SYNAPTIC AND NEURONAL DENSITY AND SYNAPSES PER NEURON
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DOI：
10.1016/0006-8993(85)90525-6
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
GREENOUGH, WT
中科院分区：
文献类型：
--
作者：
TURNER, AM;GREENOUGH, WT
大量的证据表明，不同的经验可能会导致突触数量的差异，涉及从高尔基体浸渍组织的突触后表面（棘和树突）的措施推断。高尔基体浸渍的反复无常和缺乏直接证据的传入神经的变化授权确认突触变化的EM。计算了在复合（EC）、社会（SC）或隔离（IC）环境中饲养的大鼠枕叶皮层I-IV层中每个神经元的突触比率。突触密度估计来自锇-铀酰-铅染色组织的EM，神经元密度估计来自甲苯胺蓝染色半薄切片，使用体视学方法校正突触和神经元核大小的组差异。这些密度的比率，每个神经元的突触，EC大鼠中最高，SC大鼠中居中，IC中最低。大部分差异归因于神经元密度，IC大鼠中神经元密度最高，EC中最低。突触密度在各组之间没有统计学差异。至少在这个区域和范例中，高尔基体浸渍组织中树突测量的差异反映了每个神经元突触数量的差异。
The bulk of the evidence indicating that different experiences can lead to differences in synapse numbers involves inferences from measures of postsynaptic surface (spines and dendrites) in Golgi impregnated tissue. The capriciousness of Golgi impregnation and the absence of direct evidence regarding changes in afferents mandate confirmation of synapse changes by EM. The ratio of synapses per neurons were calculated in layers I-IV of occipital cortex of rats reared in complex (EC), social (SC) or isolated (IC) environements. Synaptic density estimates were derived from EM of osmium-uranyl-lead stained tissue and neuronal density estimates were derived from toluidine blue stained semithin sections using stereological methods which correct for group differences in the sizes of synapses and neuronal nuclei. The ratio of these densities, synapses per neuron, was highest in EC rats, intermediate in SC rats and lowest in IC. The bulk of the differences were attributable to neuronal density, which was highest in IC rats and lowest in EC. Synaptic density did not differ statistically across groups. At least within this area and paradigm, the differences in dendritic measures in Golgi impregnated tissue reflect differences in the number of synapses per neuron.