Substance P inhibits the acute stimulation of ganglionic tyrosine hydroxylase activity by a nicotinic agonist.
Substance P inhibits the acute stimulation of ganglionic tyrosine hydroxylase activity by a nicotinic agonist.
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P 物质抑制烟碱激动剂对神经节酪氨酸羟化酶活性的急性刺激。
DOI：
10.1016/0306-4522(84)90271-9
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Zigmond,RE
中科院分区：
文献类型：
--
作者：
Ip,NY;Zigmond,RE
用二甲基苯基哌嗪（30μM）孵育大鼠上级颈神经节30分钟，导致酪氨酸羟化酶活性增加2倍。P物质（30 μM）可完全抑制这种作用，但不受P物质游离酸、卡生素或酸浆素的抑制。这四种肽都没有单独产生酪氨酸羟化酶活性的任何变化。P物质抑制作用的IC_（50）约为3μM。P物质不抑制氨甲酰胆碱或血管活性肠肽对该酶活性的刺激作用。因此，P物质，在一个网站，它具有不同的药理作用比以前的特点P物质受体，选择性地抑制烟碱刺激酪氨酸羟化酶activity.These数据提出的可能性，P物质可能调制烟碱调节交感神经节在体内的儿茶酚胺合成。
Incubation of rat superior cervical ganglia with dimethylphenylpiperazinium (30μM) for 30 min resulted in a two-fold increase in tyrosine hydroxylase activity. This effect was completely inhibited by substance P (30 μM) but not by substance P-free acid, kassinin or physalaemin. Neither of these four peptides alone produced any change in the activity of tyrosine hydroxylase. The IC50for the inhibitory effect of substance P was approximately 3μM. Substance P did not inhibit the stimulatory effects of bethanechol or vasoactive intestinal peptide on this enzyme activity. Thus substance P, acting at a site which has a different pharmacology than previously characterized substance P receptors, selectively inhibits nicotinic stimulation of tyrosine hydroxylase activity.These data raise the possibility that substance P may modulate the nicotinic regulation of catecholamine synthesis in sympathetic gangliain vivo.