Promoter sequences required for transcription of Xenopus laevis histone genes in injected frog oocyte nuclei.
Promoter sequences required for transcription of Xenopus laevis histone genes in injected frog oocyte nuclei.
复制标题
在注射的青蛙卵母细胞核中转录非洲爪蟾组蛋白基因所需的启动子序列。
DOI：
10.1128/mcb.8.9.3676-3682.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Perry,M
中科院分区：
文献类型：
--
作者：
Heindl,LM;Weil,TS;Perry,M
两栖动物的卵子发生伴随着组蛋白mRNA和蛋白质的积累，而不是正在进行的DNA复制。为了开始分析组蛋白基因在青蛙卵子发生和胚胎发生过程中的调控机制，我们使用卵母细胞注射检测了编码五个组蛋白类别的基因转录所需的上游序列。我们发现，最高转录水平所需的序列位于相应起始点上游的100到200个碱基对。在这个区域中，每个被研究的启动子都包含保守的序列元件，除了被认为是一般转录因子使用的其他更常见的序列元件外，其中几个似乎是组蛋白基因类特有的。
Amphibian oogenesis is accompanied by the accumulation of histone mRNA and proteins in the absence of ongoing DNA replication. To begin an analysis of the mechanisms by which histone gene expression is regulated during frog oogenesis and embryogenesis, we used oocyte injection to examine the upstream sequences required for transcription of genes encoding each of the five histone classes. We found that sequences necessary for maximal levels of transcription are located 100 to 200 base pairs upstream of the corresponding start sites. In this region, each promoter examined contains conserved sequence elements, several of which seem to be histone gene class specific, in addition to other, more common sequence elements believed to be used by general transcription factors.