A novel method for diagnosis of smear-negative tuberculosis patients by combining a random unbiased Phi29 amplification with a specific real-time PCR
A novel method for diagnosis of smear-negative tuberculosis patients by combining a random unbiased Phi29 amplification with a specific real-time PCR
复制标题
结合随机无偏 Phi29 扩增与特异性实时 PCR 诊断涂阴结核病患者的新方法
DOI：
10.1016/j.tube.2015.03.011
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发表时间：
2015-07-01
期刊：
影响因子：
3.2
通讯作者：
Zhao, Yanlin
中科院分区：
文献类型：
--
作者：
Pang, Yu;Lu, Jie;Zhao, Yanlin
In this study, we develop a novel method for diagnosis of smear-negative tuberculosis patients by performing a random unbiased Phi29 amplification prior to the use of a specific real-time PCR. The limit of detection (LOD) of the conventional real-time PCR was 100 colony-forming units (CFU) of MTB genome/reaction, while the REPLI real-time PCR assay could detect 0.4 CFU/reaction. In comparison with the conventional real-time PCR, REPLI real-time PCR shows better sensitivity for the detection of smearnegative tuberculosis (P = 0.015). (C) 2015 Published by Elsevier Ltd.
