Characteristics of Mycobacterium smegmatis J15cs strain lipids
Characteristics of Mycobacterium smegmatis J15cs strain lipids
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DOI：
10.1016/j.tube.2011.10.001
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发表时间：
2012-03-01
期刊：
影响因子：
3.2
通讯作者：
Taniguchi, Hatsumi
中科院分区：
文献类型：
--
作者：
Fujiwara, Nagatoshi;Naka, Takashi;Taniguchi, Hatsumi
耻垢分枝杆菌是一种快速生长的非致病性分枝杆菌，而M.由于其高转化率，特别是耻垢病菌株MC（2）155已被用作分枝杆菌分子分析的工具。我们检测了另一个菌株，M。smeglobulin J15cs，其具有在鼠巨噬细胞中存活6天的优势。J15cs菌株产生粗糙干燥的菌落，我们假设J15cs菌株的长期存活与其细胞壁成分相关。因此，比较了这两种菌株的脂质组成。分枝菌酸的亚类和碳种类非常相似，并且主要的糖脂和磷脂在两种菌株中表达。然而，非极性糖肽脂仅在J15cs菌株中缺失。非极性糖肽脂的存在使细胞壁具有不同的结构。此外，非极性糖肽脂通过Toll样受体2而不是4被巨噬细胞识别。我们的结论是，非极性糖肽脂的情况下是一个明确的功能的J15cs菌株，并影响其形态和在宿主细胞中的生存。(C)2011爱思唯尔有限公司版权所有。
Mycobacterium smegmatis is a rapidly growing, non-pathogenic mycobacterium, and M. smegmatis strain mc(2)155 in particular has been used as a tool for molecular analysis of mycobacteria because of its high rate of transformation. We examined another strain, M. smegmatis J15cs, which has the advantage of surviving for six days in murine macrophages. The J15cs strain produces a rough dry colony, and we hypothesized that the long survival of the J15cs strain was correlated with its cell wall components. Therefore, the lipid compositions of these two strains were compared. The subclasses and carbon species of the mycolic acids were very similar, and the major glycolipids and phospholipids were expressed in both strains. However, apolar glycopeptidolipids were deleted only in the J15cs strain. The presence of apolar glycopeptidolipids gives the cell wall a different structure. Moreover, the apolar glycopeptidolipids were recognized by macrophages via toll-like receptor 2, but not 4. We concluded that the absence of apolar glycopeptidolipids is a definitive feature of the J15cs strain, and affects its morphology and survival in host cells. (C) 2011 Elsevier Ltd. All rights reserved.