Microsatellite loci isolated from Odontesthes argentinensis and the O-perugiae species group and their use in other South American silverside fish
Microsatellite loci isolated from Odontesthes argentinensis and the O-perugiae species group and their use in other South American silverside fish
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DOI：
10.1046/j.1365-294x.2000.00882.x
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发表时间：
2000-05-01
影响因子：
4.9
通讯作者：
Sunnucks, P
中科院分区：
文献类型：
--
作者：
Beheregaray, LB;Sunnucks, P
齿形鱼（Atheriniformes：Atherinopsidae）是一种分布广泛的银鱼属，包含 15 至 22 种公认的物种，分布在南美洲温带的海洋、河口和淡水环境中（Dyer 1993）。海洋河口O. argentinensis 分布于巴西东南海岸（南纬25 度）至阿根廷丘布特省（南纬43 度）（Dyer 1993）。它是巴西、乌拉圭和阿根廷当地渔业的重要经济资源（de Buen 1953），也是适合生物测定研究的生物体（Phonlor & Cousin 1997）。淡水 O. perugiae 物种组由主要分布在巴西南部沿海平原泻湖复杂系统的形态类型组成（Dyer 1993）。我们正在使用分子标记来了解影响齿齿类进化的历史和近期生物地理情景。基于三个线粒体 DNA 基因序列的结果表明，O. argentinensis 和 O. perugiae 密切相关，并且最近在巴西南部沿海地区辐射（L. Beheregaray，未发表）。我们为这些类群开发了微卫星，以识别种群结构、澄清分类学问题并重建它们的系统发育地理学历史。提供了这些引物在各种新热带银鱼物种中应用的证据。据我们所知，这些是从南美洲本土鱼类中分离出来的第一个微卫星位点，南美洲是地球上鱼类动物种类最丰富的大陆地区之一。
Odontesthes (Atheriniformes: Atherinopsidae) is a diverse and widespread genus of silverside fish, containing between 15 and 22 recognized species distributed across marine, estuarine and freshwater environments of temperate South America (Dyer 1993). The marine-estuarine O. argentinensis ranges from the south-east coast of Brazil (25 S) to Chubut, in Argentina (43 S)(Dyer 1993). It is an economically significant resource for local fisheries in Brazil, Uruguay and Argentina (de Buen 1953) and a suitable organism for bioassay studies (Phonlor & Cousin 1997). The freshwater O. perugiae species group is composed of morphotypes mostly distributed along the complex system of lagoons of the coastal plain of southern Brazil (Dyer 1993).We are using molecular markers to understand historical and recent biogeographic scenarios that have shaped the evolution of Odontesthes. Results based on sequences of three mitochondrial DNA genes revealed that O. argentinensis and O. perugiae are closely related and have recently radiated in the coastal areas of southern Brazil (L. Beheregaray, unpublished). We developed microsatellites for these taxa to identify population structures, clarify taxonomic problems and reconstruct their phylogeographic history. Evidence for the application of these primers in a wide range of Neotropical silverside species is presented. To our knowledge, these are the first microsatellite loci isolated from fish native to South America, a continental area with one of the most diverse ichthyofaunas of the planet.