Gonadotroph-specific expression of metallothionein fusion genes in pituitaries of transgenic mice.
Gonadotroph-specific expression of metallothionein fusion genes in pituitaries of transgenic mice.
复制标题
转基因小鼠垂体中金属硫蛋白融合基因的促性腺激素特异性表达。
DOI：
10.1126/science.2868526
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Goodman,RH
中科院分区：
文献类型：
--
作者：
Low,MJ;Lechan,RM;Hammer,RE;Brinster,RL;Habener,JF;Mandel,G;Goodman,RH
表达金属硫蛋白-生长抑素融合基因的转基因小鼠在垂体前叶中含有高浓度的生长抑素，而垂体前叶通常不产生生长抑素。垂体前叶的免疫反应性生长抑素仅在促性腺激素中发现。类似地，金属硫蛋白-人生长激素融合基因也在促性腺激素中选择性表达。据推测，这两个融合基因的共同序列负责促性腺激素特异性表达。
Transgenic mice expressing a metallothionein-somatostatin fusion gene contain high concentrations of somatostatin in the anterior pituitary gland, a tissue that does not normally produce somatostatin. Immunoreactive somatostatin within the anterior pituitaries was found exclusively within gonadotrophs. Similarly, a metallothionein-human growth-hormone fusion gene was also expressed selectively in gonadotrophs. It is proposed that sequences common to the two fusion genes are responsible for the gonadotroph-specific expression.