Schistosoma mansoni: The developmental regulation and immunolocalization of antioxidant enzymes
Schistosoma mansoni: The developmental regulation and immunolocalization of antioxidant enzymes
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DOI：
10.1006/expr.1997.4150
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发表时间：
1997-05-01
影响因子：
2.1
通讯作者：
LoVerde, PT
中科院分区：
文献类型：
--
作者：
Mei, HP;LoVerde, PT
抗氧化酶。mansoni，胞质铜锌超氧化物歧化酶（CT-SOD），信号肽SOD（SP-SOD），谷胱甘肽过氧化物酶（GPX），谷胱甘肽转移酶（GST）的相对水平的转录表达进行了比较，在整个发展的半定量逆转录-聚合酶链反应测定。所有的抗氧化酶表现出类似的模式的发展调节。与幼虫阶段相比，蠕虫具有最高水平的特异性mRNA。GST表现出最高水平的表达，比CT-SOD和SP-SOD约丰富10倍，比GPX丰富100倍。这种表达的顺序几乎是一致的所有发展阶段的研究。本地化的抗氧化酶，免疫荧光染色进行3小时童虫和蠕虫。GPX，SP-SOD，CT-SOD都被发现与成人的皮层和肠上皮; SP-SOD也与细胞器和实质细胞的细胞膜，有趣的是与蠕虫的棘。另一方面，童血吸虫几乎没有免疫荧光。这些研究进一步证明了抗氧化酶的发育调节，并将其定位于宿主-寄生虫界面，支持了它们在允许蠕虫逃避免疫攻击中发挥作用的观点。(C)北京：科学出版社.
Antioxidant enzymes from S. mansoni, cytosolic Cu-Zn superoxide dismutase (CT-SOD), signal-peptide-containing SOD (SP-SOD), glutathione peroxidase (GPX), and glutathione transferase (GST) were compared for their relative levels of transcript expression throughout development in a semiquantitative reverse transcriptase-polymerase chain reaction assay. All of the antioxidant enzymes exhibited a similar pattern of developmental regulation. Adult worms have the highest level of specific mRNA compared with larval stages. GST shows the highest level of expression, being approximately 10-fold more abundant than CT-SOD and SP-SOD and 100-fold more abundant than GPX. This order of expression was nearly consistent for all the developmental stages studied. To localize the antioxidant enzymes, immunofluorescence staining was performed on 3-hr schistosomula and adult worms. GPX, SP-SOD, and CT-SOD were all found to be associated with the adult tegument and gut epithelium; SP-SOD was also associated with organelle and cell membranes of parenchymal cells and interestingly with the spines of adult worms. Schistosomula, on the other hand, showed little immunofluorescence. These studies further demonstrate the developmental regulation of antioxidant enzymes and localize them to the host-parasite interface, supporting the notion that they have a role in allowing adult worms to evade immune attack. (C) 1997 Academic Press.