Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo
Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo
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多孔碳支架的钽涂层辅以自体骨髓基质干细胞用于体外和体内骨再生
DOI：
10.1177/1535370216629578
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发表时间：
2016-03-01
影响因子：
3.2
通讯作者：
Yang, Zhenming
中科院分区：
文献类型：
--
作者：
Wei, Xiaowei;Zhao, Dewei;Yang, Zhenming
具有低弹性模量的多孔钽金属类似于松质骨。网状玻璃碳（RVC）具有三维孔结构，是钽涂层的理想支架材料。本研究首次在体外成功测试了国产多孔钽与骨髓基质干细胞（BMSCs）的生物相容性，并用于体内骨组织修复。我们使用骨髓间充质干细胞评价了RVC支架和钽涂层的细胞毒性。采用激光扫描共聚焦显微镜和扫描电镜观察BMSCs的形态、粘附和增殖情况。此外，将含有或不含有自体BMSC的多孔钽棒分别植入犬后肢。硬组织切片观察成骨能力。在植入后3周和6周，在钽-宿主骨界面和孔隙处观察到新的成骨细胞和新骨。自体骨髓间充质干细胞植入多孔钽棒12周后，钽棒孔隙内可见与成熟骨相当的再生骨小梁。结果表明，国产多孔钽具有良好的生物相容性，并能促进体内新骨形成。同时，多孔钽复合自体骨髓间充质干细胞的成骨效果优于单纯钽植入。未来的临床研究需要验证这种国产多孔钽联合自体BMSCs植入的临床疗效，并与传统的自体带血管骨移植在骨修复患者中的疗效进行比较。
Porous tantalum metal with low elastic modulus is similar to cancellous bone. Reticulated vitreous carbon (RVC) can provide three-dimensional pore structure and serves as the ideal scaffold of tantalum coating. In this study, the biocompatibility of domestic porous tantalum was first successfully tested with bone marrow stromal stem cells (BMSCs) in vitro and for bone tissue repair in vivo. We evaluated cytotoxicity of RVC scaffold and tantalum coating using BMSCs. The morphology, adhesion, and proliferation of BMSCs were observed via laser scanning confocal microscope and scanning electron microscopy. In addition, porous tantalum rods with or without autologous BMSCs were implanted on hind legs in dogs, respectively. The osteogenic potential was observed by hard tissue slice examination. At three weeks and six weeks following implantation, new osteoblasts and new bone were observed at the tantalum–host bone interface and pores. At 12 weeks postporous tantalum with autologous BMSCs implantation, regenerated trabecular equivalent to mature bone was found in the pore of tantalum rods. Our results suggested that domestic porous tantalum had excellent biocompatibility and could promote new bone formation in vivo. Meanwhile, the osteogenesis of porous tantalum associated with autologous BMSCs was more excellent than only tantalum implantation. Future clinical studies are warranted to verify the clinical efficacy of combined implantation of this domestic porous tantalum associated with autologous BMSCs implantation and compare their efficacy with conventional autologous bone grafting carrying blood vessel in patients needing bone repairing.