Novel separation mode of HPLC based on phase-separation multiphase flow
Novel separation mode of HPLC based on phase-separation multiphase flow
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DOI：
10.1007/s44211-022-00127-1
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发表时间：
2022-06-01
影响因子：
1.6
通讯作者：
Tsukagoshi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Horikawa, Kosei;Kinoshita, Satoru;Tsukagoshi, Kazuhiko
提出了一种基于相分离多相流的高效液相色谱分离方法。采用市售高效液相色谱系统，采用十八烷基二氧化硅（ODS）修饰颗粒填充分离柱。将水/乙腈/乙酸乙酯三元混合溶液(a) 1:8:1, (b) 1:3:1, (c) 16:3:1 （v/v/v）作为洗脱液在20℃和5℃下送入系统，三元混合溶液作为两相分离溶液导致相分离多相流。溶液分别在20℃和5℃下均匀和不均匀地在柱中流动。将1-萘酚（NA）和2,6-萘二磺酸（NDS）作为模型分析物注入体系。在20℃时，分析物混合物在溶液(a)和(b)中不分离，而在溶液(C)中分离，洗脱顺序为NDS先洗脱NA。在5℃时，它在溶液(a)中没有分离，而在溶液(b)中以NA→NDS的洗脱顺序和溶液(C)中以NDS→NA的洗脱顺序的分离比在20℃时更有效。这种分离行为和洗脱顺序可能是由相分离多相流引起的。
A novel separation mode for high-performance liquid chromatography (HPLC) is proposed based on phase-separation multiphase flow. A commercially available HPLC system was used with a packed-separation column of octadecyl-silica (ODS)-modified particles. Water/acetonitrile/ethyl acetate ternary mixed solutions, (a) 1:8:1, (b) 1:3:1, and (c) 16:3:1 (v/v/v), were delivered into the system as an eluent at 20 and 5 degrees C. The ternary mixed solution acted as a two-phase separation solution leading to phase-separation multiphase flow. The solution flowed in the column homogeneously and heterogeneously at 20 and 5 degrees C, respectively. 1-Naphthol (NA) and 2,6-naphthalenedisulfonic acid (NDS) were injected into the system as model analytes. At 20 degrees C, the analyte mixture did not separate in solutions (a) and (b) while it separated in solution (c) with the elution order of NDS followed by NA. At 5 degrees C, it did not separate in solution (a), while it separated in solution (b) with elution order of NA followed by NDS and solution (c) with elution order of NDS followed by NA more effectively than 20 degrees C. The separation behavior and elution order are possibly caused by the phase-separation multiphase flow.