Characterization of the Rat GAL2R Promoter: Positive Role of ETS-1 in Regulation of the Rat GAL2R Gene in PC12 Cells
Characterization of the Rat GAL2R Promoter: Positive Role of ETS-1 in Regulation of the Rat GAL2R Gene in PC12 Cells
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DOI：
10.1007/s12035-016-9986-z
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发表时间：
2016-06
影响因子：
5.1
通讯作者：
Yutao Yang;Li Liu;Hanjiang Luo;Yueting Li;Hui Li-;Z. Xu
中科院分区：
文献类型：
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作者：
Yutao Yang;Li Liu;Hanjiang Luo;Yueting Li;Hui Li-;Z. Xu
甘丙肽受体2(GAL2R)是神经肽甘丙素的G蛋白偶联受体，调节许多重要的生理功能和病理过程。为了研究GAL2R基因转录的分子机制，分离并分析了大鼠GAL2R启动子。我们发现GAL2R启动子的-320~-300区域含有两个推测的Ets-1元件，在调节GAL2R启动子活性方面起着重要作用。我们还发现转录因子Ets-1在体外和体内都与该区域结合。Ets-1过表达显著增加GAL2R启动子活性和GAL2R基因转录，而Ets-1过表达则产生相反的作用。此外，我们还发现ETS-1在GAL2R启动子中招募了共激活因子p300。这些数据表明Ets-1在调控GAL2R基因表达中起作用，并为了解GAL2R基因的转录调控提供了基础。
Galanin receptor 2 (GAL2R) is a G protein-coupled receptor for the neuropeptide galanin that regulates many important physiological functions and pathological processes. To investigate the molecular mechanism governing GAL2R gene transcription, the rat GAL2R promoter was isolated and analyzed. We found that the region from -320 to -300 of the GAL2R promoter contains two putative ETS-1 elements and plays an important role in regulating GAL2R promoter activity. We also showed that transcription factor ETS-1 bound to this region in vitro and in vivo. Overexpression of ETS-1 significantly increased GAL2R promoter activity and transcription of the GAL2R gene, whereas knockdown of ETS-1 produced the opposite effects. In addition, we showed that ETS-1 recruited co-activator p300 to the GAL2R promoter. These data indicate a role for ETS-1 in the control of the GAL2R gene expression and provide a basis for understanding the transcriptional regulation of the GAL2R gene.