Pyrethroid resistance in the tomato red spider mite, Tetranychus evansi, is associated with mutation of the para-type sodium channel
Pyrethroid resistance in the tomato red spider mite, Tetranychus evansi, is associated with mutation of the para-type sodium channel
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DOI：
10.1002/ps.2145
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发表时间：
2011-08-01
影响因子：
4.1
通讯作者：
Bass, Chris
中科院分区：
文献类型：
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作者：
Nyoni, Benjamin N.;Gorman, Kevin;Bass, Chris
背景：番茄红蜘蛛Tetranychus evansi(Baker和Pritchard)是非洲许多国家茄科作物的重要害虫。结果：马拉维的两株伊氏锥虫对有机磷毒死蜱具有耐受性，对拟除虫菊酯的抗性倍数为20-40倍，但在杀虫剂生物测定中对两种当代杀螨剂(阿维菌素和氰菊酯)敏感。从拟除虫菊酯抗性和拟除虫菊酯敏感品系中克隆了伊氏锥虫副基因的3.1kb片段(IIS5到IVS5)，发现在抗性品系中有一个非同义突变，导致通道II区域的氨基酸替换(M918T)。虽然对螨类来说是新的，但这种突变在几种昆虫中对拟除虫菊酯具有高水平的抗药性，在这些昆虫中，它总是与另一种突变(L1014F)有关。这是首次报道在节肢动物中没有L1014F的情况下M918T突变。已开发的诊断工具可以灵敏地检测到这种突变。结论：这是伊氏拟除虫菊酯对拟除虫菊酯抗性的首次研究，为马拉维南部对这种害虫的抗性管理提供了当代信息。(C)2011年化学工业学会
BACKGROUND: The tomato red spider mite, Tetranychus evansi (Baker and Pritchard), is a serious pest of solanaceous crops in many African countries. In this study an investigation has been conducted to establish whether mutation of the para-type sodium channel underlies pyrethroid resistance in T. evansi strains collected in Southern Malawi.RESULTS: Two T. evansi strains from Malawi showed tolerance to the organophosphate chlorpyrifos and resistance (20-40-fold) to the pyrethroid bifenthrin, but were susceptible to two contemporary acaricides (abamectin and fenpyroximate) in insecticide bioassays. Cloning of a 3.1 kb fragment (domains IIS5 to IVS5) of the T. evansi para gene from pyrethroid-resistant and pyrethroid-susceptible strains revealed a single non-synonymousmutation in the resistant strains that results in an amino acid substitution (M918T) within the domain II region of the channel. Although novel to mites, this mutation confers high levels of resistance to pyrethroids in several insect species where it has always been associated with another mutation (L1014F). This is the first report of the M918T mutation in the absence of L1014F in any arthropod species. Diagnostic tools were developed that allow sensitive detection of this mutation in individual mites.CONCLUSION: This is the first study of pyrethroid resistance in T. evansi and provides contemporary information for resistance management of this pest in Southern Malawi. (C) 2011 Society of Chemical Industry