Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion
Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion
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DOI：
10.1006/jsbi.2001.4406
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发表时间：
2001-09-01
影响因子：
3
通讯作者：
Hegerl, R
中科院分区：
文献类型：
--
作者：
Frangakis, AS;Hegerl, R
电子断层摄影术是一种强大的技术，能够提供独特的见解到多形性生物体的三维结构组织。然而，所得到的体积数据的可视化和解释受到极低的信噪比的阻碍，特别是当冰包埋的生物标本进行调查。通常，这种数据的等值面表示或体绘制在没有任何进一步的信号增强的情况下受到阻碍。提出了一种基于非线性各向异性扩散的降噪方法。该方法将有效的降噪与出色的信号保留相结合，并且明显上级传统方法（例如，低通和中值滤波）和不变小波变换滤波。通过傅里叶壳相关分析，验证了信噪比的增益。处理后的3D图像的可视化性能提高证明了两个例子，层析重建的染色质和一个candidon。详细介绍了参数设置和离散格式。(C)2001年，爱思唯尔科学。
Electron tomography is a powerful technique capable of giving unique insights into the three-dimensional structural organization of pleomorphic biological objects. However, visualization and interpretation of the resulting volumetric data are hampered by an extremely low signal-to-noise ratio, especially when ice-embedded biological specimens are investigated. Usually, isosurface representation or volume rendering of such data is hindered without any further signal enhancement. We propose a novel technique for noise reduction based on nonlinear anisotropic diffusion. The approach combines efficient noise reduction with excellent signal preservation and is clearly superior to conventional methods (e.g., low-pass and median filtering) and invariant wavelet transform filtering. The gain in the signal-to-noise ratio is verified and demonstrated by means of Fourier shell correlation. Improved visualization performance after processing the 3D images is demonstrated with two examples, tomographic reconstructions of chromatin and of a mitochondrion. Parameter settings and discretization stencils are presented in detail. (C) 2001 Elsevier Science.