Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen
Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen
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DOI：
10.1016/s0264-410x(03)00360-8
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发表时间：
2003-09-08
期刊：
影响因子：
5.5
通讯作者：
Yang, MS
中科院分区：
文献类型：
--
作者：
Bae, JL;Lee, JG;Yang, MS
猪中乳酸免疫的报告保护了乳囊免受猪流行腹泻病毒（PEDV）的感染，这表明在泌乳猪中诱导粘膜免疫反应是保护猪免受PEDV感染的有效方法。在这项研究中，我们开发了表达与佩德维（Pedv）尖峰蛋白中和表位相对应的抗原蛋白的转基因烟草植物，并测试了将植物喂食到猪中是否诱导了针对PEDV感染的有效免疫反应。首先，我们通过使用将小鼠从转基因植物中提取的蛋白质中获得的血清中和血清，证实了植物来源的抗原的免疫原性。将转基因植物喂入小鼠诱导抗原的全身和粘膜免疫反应。诱导的抗体抑制了斑块还原中和测定中的病毒感染。这些结果表明，饲养动物的转基因植物携带抗原基因是一种有效的策略，可以诱导针对PEDV感染的保护性免疫反应。 （c）2003 Elsevier Science Ltd.保留所有权利。
A report from that the presence of lactogenic immunity in pigs protected suckling piglets from porcine epidemic diarrhea virus (PEDV) infection suggested that inducing mucosal immune responses in lactating pigs is an effective way of protecting swine from PEDV infection. In this study, we developed transgenic tobacco plants that express the antigen protein corresponding to the neutralizing epitope of PEDV spike protein, and tested whether feeding the plants to pigs induced an effective immune response against PEDV infection. First, we confirmed the immunogenicity of the plant-derived antigen by using a plaque reduction neutralization assay with serum obtained after injecting mice with protein extracted from the transgenic plants. Feeding the transgenic plants to mice induced both systemic and mucosal immune responses against the antigen. The induced antibodies inhibited virus infection in the plaque reduction neutralization assay. These results suggest that feeding animals transgenic plants carrying antigen genes is an effective strategy to induce protective immune responses against PEDV infection. (C) 2003 Elsevier Science Ltd. All rights reserved.