Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells.

Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells.
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循环肿瘤细胞的计数、介电泳捕获和分子分析。

DOI:
10.1007/978-1-4939-7144-2_16
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发表时间:
2017
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Carpenter,EricaL
Carpenter,EricaL
中科院分区:
--
文献类型:
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作者:
Yee,StephanieS;Carpenter,EricaL

文献摘要

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从癌症患者血液中鉴定循环肿瘤细胞(CTC)中的治疗靶向突变越来越多地用于个性化患者护理。在这里,我们描述了一种新的方法,用于从血液中计数,捕获和分子分析CTC,使用FDA批准的CTC富集和计数平台,然后进行介电泳捕获和下一代测序。
The identification of therapeutically targetable mutations in circulating tumor cells (CTCs) from cancer patient blood is increasingly used to personalize patient care. Here, we describe a novel approach for the enumeration, capture, and molecular analysis of CTCs from blood using an FDA-approved CTC enrichment and enumeration platform followed by dielectrophoretic capture and next-generation sequencing.