ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid.
ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid.
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DOI：
10.1046/j.1365-313x.1998.00231.x
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发表时间：
1998-08
期刊：
影响因子：
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通讯作者：
Hong Wang;Hong Wang;Q. Qi;P. Schorr;A. Cutler;W. Crosby;L. Fowke
中科院分区：
文献类型：
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作者：
Hong Wang;Hong Wang;Q. Qi;P. Schorr;A. Cutler;W. Crosby;L. Fowke
细胞周期蛋白依赖性激酶（CDK）抑制剂基因编码的低分子量蛋白在细胞周期调控，发展和可能也在肿瘤发生中具有重要的功能。第一个植物CDK抑制剂基因ICK 1是最近从拟南芥中发现的。尽管ICK 1的C-末端结构域包含与哺乳动物CDK抑制剂p27 Kip 1的重要共有序列，但推断的ICK 1序列的其余部分与任何已知的CDK抑制剂几乎没有相似性。体外实验表明，重组ICK 1具有独特的激酶抑制特性。在本研究中，我们根据ICK 1的基因结构，它与A。拟南芥Cdc 2a和CycD 3的表达，以及植物生长调节剂脱落酸（阿坝）对该表达的诱导。在所调查的组织中，ICK 1的表达水平相对较低。然而，ICK 1被阿坝诱导，并且沿着ICK 1诱导，Cdc 2样组蛋白H1激酶活性降低。这些结果表明阿坝抑制植物细胞分裂的分子机制。ICK 1克隆也从使用CycD 3构建体的独立酵母双杂交筛选中鉴定。ICK 1蛋白可以与Cdc 2a和CycD 3相互作用的暗示通过体外结合试验被证实。此外，缺失分析表明，ICK 1的不同区域需要与Cdc 2a和CycD 3的相互作用。这些结果为理解CDK抑制剂在植物细胞周期调控中的作用提供了机制基础。
Cyclin-dependent kinase (CDK) inhibitor genes encode low molecular weight proteins which have important functions in cell cycle regulation, development and perhaps also in tumorigenesis. The first plant CDK inhibitor gene ICK1 was recently identified from Arabidopsis thaliana. Although the C-terminal domain of ICK1 contained an important consensus sequence with the mammalian CDK inhibitor p27Kip1, the remainder of the deduced ICK1 sequence showed little similarity to any known CDK inhibitors. In vitro assays showed that recombinant ICK1 exhibited unique kinase inhibitory properties. In the present study we characterized ICK1 in terms of its gene structure, its interaction with both A. thaliana Cdc2a and CycD3, and its induction by the plant growth regulator, abscisic acid (ABA). ICK1 was expressed at a relatively low level in the tissues surveyed. However, ICK1 was induced by ABA, and along with ICK1 induction there was a decrease in Cdc2-like histone H1 kinase activity. These results suggest a molecular mechanism by which plant cell division might be inhibited by ABA. ICK1 clones were also identified from independent yeast two-hybrid screens using the CycD3 construct. The implication that ICK1 protein could interact with both Cdc2a and CycD3 was confirmed by in vitro binding assays. Furthermore, deletion analysis indicated that different regions of ICK1 are required for the interactions with Cdc2a and CycD3. These results provide a mechanistic basis for understanding the role of CDK inhibitors in cell cycle regulation in plant cells.